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Literature summary extracted from

  • Bareich, D.; Koteva, K.; Nazi, I.; Wright, G.D.
    Small molecule functional discrimination of the kinases required for the microbial synthesis of threonine and isoleucine (2004), Bioorg. Med. Chem., 12, 807-815.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.39 medicine potential target for antibiotics due to the fact, that the pathway is not found in mammals Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.39 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.27
-
 ATP pH 7.5, 30°C Schizosaccharomyces pombe
2.7.1.39 0.52
-
 L-homoserine pH 7.5, 30°C Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.39 ATP + L-homoserine Schizosaccharomyces pombe involved in the biosynthesis of threonine and isoleucine ADP + O-phospho-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.39 recombinant enzyme using His-tag Schizosaccharomyces pombe

Storage Stability

EC Number Storage Stability Organism
2.7.1.39 -80°C, 20 mM HEPES, pH 8.0, 10% glycerol, storage of purified enzyme Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?
2.7.1.39 ATP + L-homoserine involved in the biosynthesis of threonine and isoleucine Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.39 40.2
-
 L-homoserine pH 7.5, 30°C Schizosaccharomyces pombe
2.7.1.39 40.8
-
 ATP pH 7.5, 30°C Schizosaccharomyces pombe