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Literature summary extracted from

  • Zhou, L.; Marks, T.S.; Poh, R.P.; Smith, R.J.; Chowdhry, B.Z.; Smith, A.R.
    The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1 (2004), Biodegradation, 15, 97-109.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.7 0.009
-
 4-chlorobenzoyl-CoA 30°C, pH 7.5 Arthrobacter sp.
6.2.1.33 0.0035
-
 4-Chlorobenzoate 25°C, pH 7.0 Arthrobacter sp.
6.2.1.33 0.0238
-
 ATP 25°C, pH 7.0 Arthrobacter sp.
6.2.1.33 0.03
-
 CoA 25°C, pH 7.0 Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.7 33000
-
 4 * 33000, SDS-PAGE Arthrobacter sp.
3.8.1.7 131000
-
 gel filtration Arthrobacter sp.
6.2.1.33 56000
-
 2 * 56000, SDS-PAGE Arthrobacter sp.
6.2.1.33 112000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.7 Arthrobacter sp.
-
-
-
3.8.1.7 Arthrobacter sp. TM-1
-
-
-
6.2.1.33 Arthrobacter sp.
-
-
-
6.2.1.33 Arthrobacter sp. TM-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.7
-
 Arthrobacter sp.
6.2.1.33
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.7 3.61
-
 pH 7.5, 30°C Arthrobacter sp.
6.2.1.33 0.86
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Arthrobacter sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Arthrobacter sp. TM-1 4-hydroxybenzoyl-CoA + chloride
-
 ?
6.2.1.33 4-Bromobenzoate + CoA + ATP 93% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Bromobenzoate + CoA + ATP 93% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Arthrobacter sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Arthrobacter sp. TM-1 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Fluorobenzoate + CoA + ATP 67% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Fluorobenzoate + CoA + ATP 67% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Iodobenzoate + CoA + ATP 73% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Iodobenzoate + CoA + ATP 73% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 Benzoate + CoA + ATP 62% of the activity with 4-chlorobenzoate Arthrobacter sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.7 tetramer 4 * 33000, SDS-PAGE Arthrobacter sp.
6.2.1.33 dimer 2 * 56000, SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
6.2.1.33 4-CB:CoA ligase
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.7 45
-
-
 Arthrobacter sp.
6.2.1.33 25
-
-
 Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.7 15 55 15°C: about 50% of maximal activity, 55°C: about 50% of maximal activity Arthrobacter sp.
6.2.1.33 10 40 10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity Arthrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.7 1
-
 4-chlorobenzoyl-CoA 30°C, pH 7.5 Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.7 7.5
-
-
 Arthrobacter sp.
6.2.1.33 7
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.7 6 9.3 pH 6.0: about 55% of maximal activity, pH 9.3: about 55% of maximal activity Arthrobacter sp.
6.2.1.33 5.8 8.2 pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity Arthrobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.7 Arthrobacter sp. isoelectric focusing
-
 6.4
6.2.1.33 Arthrobacter sp.
-
-
 5.8