BRENDA - Enzyme Database

The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1

Zhou, L.; Marks, T.S.; Poh, R.P.; Smith, R.J.; Chowdhry, B.Z.; Smith, A.R.; Biodegradation 15, 97-109 (2004)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.009
-
4-chlorobenzoyl-CoA
30°C, pH 7.5
Arthrobacter sp.
6.2.1.33
0.0035
-
4-Chlorobenzoate
25°C, pH 7.0
Arthrobacter sp.
6.2.1.33
0.0238
-
ATP
25°C, pH 7.0
Arthrobacter sp.
6.2.1.33
0.03
-
CoA
25°C, pH 7.0
Arthrobacter sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.8.1.7
33000
-
4 * 33000, SDS-PAGE
Arthrobacter sp.
3.8.1.7
131000
-
gel filtration
Arthrobacter sp.
6.2.1.33
56000
-
2 * 56000, SDS-PAGE
Arthrobacter sp.
6.2.1.33
112000
-
gel filtration
Arthrobacter sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.8.1.7
Arthrobacter sp.
-
-
-
3.8.1.7
Arthrobacter sp. TM-1
-
-
-
6.2.1.33
Arthrobacter sp.
-
-
-
6.2.1.33
Arthrobacter sp. TM-1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.8.1.7
-
Arthrobacter sp.
6.2.1.33
-
Arthrobacter sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8.1.7
3.61
-
pH 7.5, 30°C
Arthrobacter sp.
6.2.1.33
0.86
-
-
Arthrobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
661288
Arthrobacter sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
661288
Arthrobacter sp. TM-1
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
6.2.1.33
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp. TM-1
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
Benzoate + CoA + ATP
62% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.8.1.7
tetramer
4 * 33000, SDS-PAGE
Arthrobacter sp.
6.2.1.33
dimer
2 * 56000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.8.1.7
45
-
-
Arthrobacter sp.
6.2.1.33
25
-
-
Arthrobacter sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.8.1.7
15
55
15°C: about 50% of maximal activity, 55°C: about 50% of maximal activity
Arthrobacter sp.
6.2.1.33
10
40
10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity
Arthrobacter sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
1
-
4-chlorobenzoyl-CoA
30°C, pH 7.5
Arthrobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
7.5
-
-
Arthrobacter sp.
6.2.1.33
7
-
-
Arthrobacter sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.8.1.7
6
9.3
pH 6.0: about 55% of maximal activity, pH 9.3: about 55% of maximal activity
Arthrobacter sp.
6.2.1.33
5.8
8.2
pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity
Arthrobacter sp.
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.8.1.7
Arthrobacter sp.
isoelectric focusing
-
6.4
6.2.1.33
Arthrobacter sp.
-
-
5.8
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.8.1.7
0.009
-
4-chlorobenzoyl-CoA
30°C, pH 7.5
Arthrobacter sp.
6.2.1.33
0.0035
-
4-Chlorobenzoate
25°C, pH 7.0
Arthrobacter sp.
6.2.1.33
0.0238
-
ATP
25°C, pH 7.0
Arthrobacter sp.
6.2.1.33
0.03
-
CoA
25°C, pH 7.0
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.8.1.7
33000
-
4 * 33000, SDS-PAGE
Arthrobacter sp.
3.8.1.7
131000
-
gel filtration
Arthrobacter sp.
6.2.1.33
56000
-
2 * 56000, SDS-PAGE
Arthrobacter sp.
6.2.1.33
112000
-
gel filtration
Arthrobacter sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.8.1.7
-
Arthrobacter sp.
6.2.1.33
-
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8.1.7
3.61
-
pH 7.5, 30°C
Arthrobacter sp.
6.2.1.33
0.86
-
-
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
661288
Arthrobacter sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
3.8.1.7
4-chlorobenzoyl-CoA + H2O
-
661288
Arthrobacter sp. TM-1
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
6.2.1.33
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Bromobenzoate + CoA + ATP
93% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Bromobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp.
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Chlorobenzoate + CoA + ATP
-
661288
Arthrobacter sp. TM-1
4-Chlorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Fluorobenzoate + CoA + ATP
67% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Fluorobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
4-Iodobenzoate + CoA + ATP
73% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp. TM-1
4-Iodobenzoyl-CoA + AMP + diphosphate
-
-
-
?
6.2.1.33
Benzoate + CoA + ATP
62% of the activity with 4-chlorobenzoate
661288
Arthrobacter sp.
Benzoyl-CoA + AMP + diphosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.8.1.7
tetramer
4 * 33000, SDS-PAGE
Arthrobacter sp.
6.2.1.33
dimer
2 * 56000, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.8.1.7
45
-
-
Arthrobacter sp.
6.2.1.33
25
-
-
Arthrobacter sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.8.1.7
15
55
15°C: about 50% of maximal activity, 55°C: about 50% of maximal activity
Arthrobacter sp.
6.2.1.33
10
40
10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity
Arthrobacter sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8.1.7
1
-
4-chlorobenzoyl-CoA
30°C, pH 7.5
Arthrobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8.1.7
7.5
-
-
Arthrobacter sp.
6.2.1.33
7
-
-
Arthrobacter sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.8.1.7
6
9.3
pH 6.0: about 55% of maximal activity, pH 9.3: about 55% of maximal activity
Arthrobacter sp.
6.2.1.33
5.8
8.2
pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity
Arthrobacter sp.
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.8.1.7
Arthrobacter sp.
isoelectric focusing
-
6.4
6.2.1.33
Arthrobacter sp.
-
-
5.8