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Literature summary extracted from

  • Santa, H.; Kammonen, J.; Lehtonen, O.; Karimaki, J.; Pastinen, O.; Leisola, M.; Turunen, O.
    Stochastic boundary molecular dynamics simulation of L-ribose in the active site of Actinoplanes missouriensis xylose isomerase and its Val135Asn mutant with improved reaction rate (2005), Biochim. Biophys. Acta, 1749, 65-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in Escherichia coli Actinoplanes missouriensis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 V135N no effect on the reaction with D-xylose and L-arabinose, reaction efficiency with L-ribose is increased 2-4fold, reaction with D-glucose is impaired Actinoplanes missouriensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 244
-
 D-glucose mutant V135N Actinoplanes missouriensis
5.3.1.5 438
-
 L-ribose mutant V135N Actinoplanes missouriensis
5.3.1.5 713
-
 D-glucose wild-type Actinoplanes missouriensis
5.3.1.5 1312
-
 L-ribose wild-type Actinoplanes missouriensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Actinoplanes missouriensis
-
 D-Xylulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Actinoplanes missouriensis P12851
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5 recombinant enzyme, by heat precipitation and cation exchange, proteins were 95% pure when estimated in SDS-PAGE Actinoplanes missouriensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Actinoplanes missouriensis D-Fructose
-
 ?
5.3.1.5 D-ribose
-
 Actinoplanes missouriensis D-ribulose
-
 r
5.3.1.5 D-ribose
-
 Actinoplanes missouriensis L-arabinose
-
 r
5.3.1.5 D-Xylose
-
 Actinoplanes missouriensis D-Xylulose
-
 ?
5.3.1.5 L-arabinose
-
 Actinoplanes missouriensis D-ribose
-
 r
5.3.1.5 L-arabinose
-
 Actinoplanes missouriensis D-ribulose
-
 r
5.3.1.5 L-ribose
-
 Actinoplanes missouriensis L-ribulose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 XI
-
 Actinoplanes missouriensis
5.3.1.5 xylose isomerase
-
 Actinoplanes missouriensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 0.0226
-
 L-ribose mutant V135N Actinoplanes missouriensis
5.3.1.5 0.0252
-
 L-ribose wild-type Actinoplanes missouriensis
5.3.1.5 3.47
-
 D-glucose mutant V135N Actinoplanes missouriensis
5.3.1.5 26.3
-
 D-glucose wild-type Actinoplanes missouriensis