BRENDA - Enzyme Database show

Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase

Yeh, H.C.; Hsu, P.Y.; Wang, J.S.; Tsai, A.L.; Wang, L.H.; Biochim. Biophys. Acta 1738, 121-132 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.4
expression in Escherichia coli
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.0133
-
prostaglandin H2
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
membrane
the recombinant enzyme associates with host cell membranes
Homo sapiens
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Homo sapiens
-
Prostaglandin I2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.4
to electrophoretic homogeneity
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.4
46
-
CM column, assayed at 23░C using 30 microM prostaglandin H1 as the substrate
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
10-hydroperoxyoctadeca-8,12-dienoic acid
-
661260
Homo sapiens
10-oxooctadeca-8,12-dienoic acid + 10-oxodec-8-enoic acid + 10-hydroxyoctadeca-8,12-dienoic acid
-
-
-
?
5.3.99.4
prostaglandin H1
-
661260
Homo sapiens
12-hydroxy-8,10-heptadecadienoic acid + malondialdehyde
-
-
-
?
5.3.99.4
Prostaglandin H2
-
661260
Homo sapiens
Prostaglandin I2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.4
heme
heme/protein ratio is 0.9:1
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
expression in Escherichia coli
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.4
heme
heme/protein ratio is 0.9:1
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.0133
-
prostaglandin H2
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
membrane
the recombinant enzyme associates with host cell membranes
Homo sapiens
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Homo sapiens
-
Prostaglandin I2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
to electrophoretic homogeneity
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.4
46
-
CM column, assayed at 23░C using 30 microM prostaglandin H1 as the substrate
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
10-hydroperoxyoctadeca-8,12-dienoic acid
-
661260
Homo sapiens
10-oxooctadeca-8,12-dienoic acid + 10-oxodec-8-enoic acid + 10-hydroxyoctadeca-8,12-dienoic acid
-
-
-
?
5.3.99.4
prostaglandin H1
-
661260
Homo sapiens
12-hydroxy-8,10-heptadecadienoic acid + malondialdehyde
-
-
-
?
5.3.99.4
Prostaglandin H2
-
661260
Homo sapiens
Prostaglandin I2
-
-
-
?