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Literature summary extracted from

  • Kezmarsky, N.D.; Xu, H.; Graham, D.E.; White, R.H.
    Identification and characterization of a L-tyrosine decarboxylase in Methanocaldococcus jannaschii (2005), Biochim. Biophys. Acta, 1722, 175-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.25 transformed into Escherichia coli BL21(DE3) Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.25 hydroxylamine 2 mM, complete inhibition Methanocaldococcus jannaschii
4.1.1.25 O-Methylhydroxylamine 2 mM, complete inhibition Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.25 1.6
-
 L-tyrosine
-
 Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.25 45000
-
 2 * 45000, SDS-PAGE Methanocaldococcus jannaschii
4.1.1.25 90000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.25 Methanocaldococcus jannaschii Q60358
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.25
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.25 L-aspartate 95% of the activity with L-tyrosine Methanocaldococcus jannaschii 3-aminopropionic acid + CO2
-
 ?
4.1.1.25 L-glutamate 80% of the activity with L-tyrosine Methanocaldococcus jannaschii 4-aminobutyric acid + CO2
-
 ?
4.1.1.25 L-Tyrosine
-
 Methanocaldococcus jannaschii Tyramine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.25 dimer 2 * 45000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.1.1.25 MfnA protein
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.25 100
-
 10 min, enzyme retains full activity Methanocaldococcus jannaschii
4.1.1.25 110
-
 10 min, 68% loss of activity Methanocaldococcus jannaschii
4.1.1.25 121
-
 10 min, complete loss of activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.25 7.5 8.5
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.25 pyridoxal 5'-phosphate dependent on Methanocaldococcus jannaschii