Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Leang, K.; Takada, G.; Fukai, Y.; Morimoto, K.; Granstrom, T.B.; Izumori, K.
    Novel reactions of L-rhamnose isomerase from Pseudomonas stutzeri and its relation with D-xylose isomerase via substrate specificity (2004), Biochim. Biophys. Acta, 1674, 68-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 overexpression in Escherichia coli Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.14 Ag+ 1 mM, complete inhibition Pseudomonas stutzeri
5.3.1.14 Ba2+ 1 mM, 97% inhibition Pseudomonas stutzeri
5.3.1.14 Ca2+ 1 mM, 88% inhibition Pseudomonas stutzeri
5.3.1.14 Co2+ 1 mM, 79% inhibition Pseudomonas stutzeri
5.3.1.14 Cu2+ 1 mM, 61% inhibition Pseudomonas stutzeri
5.3.1.14 EDTA 1 mM, 98% inhibition Pseudomonas stutzeri
5.3.1.14 Fe2+ 1 mM, complete inhibition Pseudomonas stutzeri
5.3.1.14 Mg2+ 1 mM, 45% inhibition Pseudomonas stutzeri
5.3.1.14 Na+ 1 mM, 12% inhibition Pseudomonas stutzeri
5.3.1.14 Ni2+ 1 mM, 97% inhibition Pseudomonas stutzeri
5.3.1.14 Zn2+ 1 mM, 89% inhibition Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 11
-
 L-rhamnulose
-
 Pseudomonas stutzeri
5.3.1.14 38
-
 D-ribose
-
 Pseudomonas stutzeri
5.3.1.14 42
-
 D-allose
-
 Pseudomonas stutzeri
5.3.1.14 54
-
 L-Mannose
-
 Pseudomonas stutzeri
5.3.1.14 61
-
 L-Lyxose
-
 Pseudomonas stutzeri
5.3.1.14 71
-
 D-altrose
-
 Pseudomonas stutzeri
5.3.1.14 127
-
 D-arabinose
-
 Pseudomonas stutzeri
5.3.1.14 202
-
 L-Xylose
-
 Pseudomonas stutzeri
5.3.1.14 250
-
 D-xylose
-
 Pseudomonas stutzeri
5.3.1.14 564
-
 D-glucose
-
 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Al3+ 1 mM, activates to 104% of control Pseudomonas stutzeri
5.3.1.14 Li+ 1 mM, activates to 106% of control Pseudomonas stutzeri
5.3.1.14 Mn2+ 1 mM, activates to 112% of control Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 43000
-
 x * 43000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Pseudomonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 recombinant His-tagged enzyme Pseudomonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 additional information
-
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Pseudomonas stutzeri D-psicose + D-altrose
-
 ?
5.3.1.14 D-altrose
-
 Pseudomonas stutzeri D-psicose + D-altrose
-
 r
5.3.1.14 D-arabinose
-
 Pseudomonas stutzeri D-ribulose + D-ribose
-
 r
5.3.1.14 D-erythrose
-
 Pseudomonas stutzeri D-erythrulose + D-threose
-
 r
5.3.1.14 D-galactose
-
 Pseudomonas stutzeri D-tagatose
-
 r
5.3.1.14 D-glucose
-
 Pseudomonas stutzeri D-fructose + D-mannose
-
 r
5.3.1.14 D-gulose
-
 Pseudomonas stutzeri D-sorbose
-
 r
5.3.1.14 D-lyxose
-
 Pseudomonas stutzeri D-xylulose + D-xylose
-
 r
5.3.1.14 D-ribose
-
 Pseudomonas stutzeri D-ribulose + D-arabinose
-
 r
5.3.1.14 D-threose
-
 Pseudomonas stutzeri D-erythrulose + D-erythrose
-
 r
5.3.1.14 D-xylose
-
 Pseudomonas stutzeri D-xylulose + D-lyxose
-
 r
5.3.1.14 L-arabinose
-
 Pseudomonas stutzeri L-ribulose
-
 r
5.3.1.14 L-erythrose
-
 Pseudomonas stutzeri L-erythrulose + L-threose
-
 r
5.3.1.14 L-galactose
-
 Pseudomonas stutzeri L-tagatose + L-talose
-
 r
5.3.1.14 L-glucose
-
 Pseudomonas stutzeri L-fructose + L-mannose
-
 r
5.3.1.14 L-lyxose
-
 Pseudomonas stutzeri L-xylulose + L-xylose
-
 r
5.3.1.14 L-mannose
-
 Pseudomonas stutzeri L-fructose + L-glucose
-
 r
5.3.1.14 L-rhamnulose
-
 Pseudomonas stutzeri L-rhamnulose
-
 r
5.3.1.14 L-ribose
-
 Pseudomonas stutzeri L-ribulose + L-arabinose
-
 r
5.3.1.14 L-threose
-
 Pseudomonas stutzeri L-erythrulose + L-erythrose
-
 r
5.3.1.14 L-xylose
-
 Pseudomonas stutzeri L-xylulose + L-lyxose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 43000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 0.017
-
 L-Xylose
-
 Pseudomonas stutzeri
5.3.1.14 0.024
-
 D-glucose
-
 Pseudomonas stutzeri
5.3.1.14 0.05
-
 D-altrose
-
 Pseudomonas stutzeri
5.3.1.14 0.17
-
 D-arabinose
-
 Pseudomonas stutzeri
5.3.1.14 33
-
 D-xylose
-
 Pseudomonas stutzeri
5.3.1.14 900
-
 L-Lyxose
-
 Pseudomonas stutzeri
5.3.1.14 1000
-
 L-Mannose
-
 Pseudomonas stutzeri
5.3.1.14 1717
-
 L-rhamnulose
-
 Pseudomonas stutzeri
5.3.1.14 2500
-
 D-allose
-
 Pseudomonas stutzeri
5.3.1.14 3150
-
 D-ribose
-
 Pseudomonas stutzeri