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Literature summary extracted from

  • Strisovsky, K.; Jiraskova, J.; Mikulova, A.; Rulisek, L.; Konvalinka, J.
    Dual substrate and reaction specificity in mouse serine racemase: identification of high-affinity dicarboxylate substrate and inhibitors and analysis of the beta-eliminase activity (2005), Biochemistry, 44, 13091-13100.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.18 Dihydroxyfumarate
-
 Mus musculus
5.1.1.18 glycine competitive Mus musculus
5.1.1.18 L-asparagine
-
 Mus musculus
5.1.1.18 L-cysteine-S-sulfate
-
 Mus musculus
5.1.1.18 L-erythro-3-hydroxyaspartate
-
 Mus musculus
5.1.1.18 Maleate
-
 Mus musculus
5.1.1.18 malonate
-
 Mus musculus
5.1.1.18 meso-tartrate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 0.49
-
 L-serine O-sulfate pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 3.2
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
4.3.1.17 4
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.10 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.10 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.18 0.49
-
 L-serine O-sulfate pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 3.2
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.18 4
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 36121
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
4.3.1.17 36123
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.10 36121
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.10 36123
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.18 36121
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.18 36123
-
 x * 36121, MALDI-MS, x * 36123, calculated Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Mus musculus Q9QZX7 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.10 Mus musculus
-
 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.18 Mus musculus Q9QZX7 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 beta-chloro-L-alanine
-
 Mus musculus 3-chloropropenoic acid + NH3
-
 ?
4.3.1.17 D-serine elimination reaction Mus musculus pyruvate + NH3
-
 ?
4.3.1.17 D-serine racemization reaction Mus musculus L-serine
-
 r
4.3.1.17 L-serine elimination reaction Mus musculus pyruvate + NH3
-
 ?
4.3.1.17 L-serine racemization reaction Mus musculus D-serine
-
 r
4.3.1.17 L-serine O-sulfate elimination reaction Mus musculus O-sulfopyruvate + NH3
-
 ?
4.3.1.17 L-threo-3-hydroxyaspartate
-
 Mus musculus oxaloacetate + NH3
-
 ?
4.3.1.17 L-threonine
-
 Mus musculus 2-oxobutyrate + NH3
-
 ?
4.3.1.17 additional information racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon Mus musculus ?
-
 ?
5.1.1.10 D-serine
-
 Mus musculus L-serine
-
 r
5.1.1.10 L-serine
-
 Mus musculus D-serine
-
 r
5.1.1.10 additional information racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon Mus musculus ?
-
 ?
5.1.1.18 D-serine
-
 Mus musculus pyruvate + NH3
-
 ?
5.1.1.18 D-serine
-
 Mus musculus L-serine
-
 r
5.1.1.18 L-serine
-
 Mus musculus pyruvate + NH3
-
 ?
5.1.1.18 L-serine
-
 Mus musculus D-serine
-
 r
5.1.1.18 L-serine O-sulfate
-
 Mus musculus O-sulfopyruvate + NH3
-
 ?
5.1.1.18 additional information racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.17 ? x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.10 ? x * 36121, MALDI-MS, x * 36123, calculated Mus musculus
5.1.1.18 ? x * 36121, MALDI-MS, x * 36123, calculated Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.17 0.49
-
 L-serine O-sulfate pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 2.6
-
 beta-chloro-L-alanine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 3.2
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
4.3.1.17 4
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 10.5
-
 L-threonine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
4.3.1.17 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
4.3.1.17 31
-
 L-threo-3-hydroxyaspartate pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.10 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.10 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.18 0.49
-
 L-serine O-sulfate pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 3.2
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 3.8
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus
5.1.1.18 4
-
 L-serine pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction Mus musculus
5.1.1.18 14.5
-
 D-serine pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.18 0.043
-
 L-erythro-3-hydroxyaspartate pH 8.0, 37°C Mus musculus
5.1.1.18 0.071
-
 malonate pH 8.0, 37°C Mus musculus
5.1.1.18 0.55
-
 Maleate pH 8.0, 37°C Mus musculus
5.1.1.18 0.64
-
 L-cysteine-S-sulfate pH 8.0, 37°C Mus musculus
5.1.1.18 0.66
-
 meso-tartrate pH 8.0, 37°C Mus musculus
5.1.1.18 0.69
-
 Dihydroxyfumarate pH 8.0, 37°C Mus musculus
5.1.1.18 1.13
-
 L-asparagine pH 8.0, 37°C Mus musculus
5.1.1.18 1.64
-
 glycine pH 8.0, 37°C Mus musculus