BRENDA - Enzyme Database

Dual substrate and reaction specificity in mouse serine racemase: identification of high-affinity dicarboxylate substrate and inhibitors and analysis of the beta-eliminase activity

Strisovsky, K.; Jiraskova, J.; Mikulova, A.; Rulisek, L.; Konvalinka, J.; Biochemistry 44, 13091-13100 (2005)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.18
Dihydroxyfumarate
-
Mus musculus
5.1.1.18
glycine
competitive
Mus musculus
5.1.1.18
L-asparagine
-
Mus musculus
5.1.1.18
L-cysteine-S-sulfate
-
Mus musculus
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
Maleate
-
Mus musculus
5.1.1.18
malonate
-
Mus musculus
5.1.1.18
meso-tartrate
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.17
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.17
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
4.3.1.17
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.17
Mus musculus
Q9QZX7
bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.10
Mus musculus
-
bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.18
Mus musculus
Q9QZX7
bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.17
beta-chloro-L-alanine
-
661171
Mus musculus
3-chloropropenoic acid + NH3
-
-
-
?
4.3.1.17
D-serine
elimination reaction
661171
Mus musculus
pyruvate + NH3
-
-
-
?
4.3.1.17
D-serine
racemization reaction
661171
Mus musculus
L-serine
-
-
-
r
4.3.1.17
L-serine
elimination reaction
661171
Mus musculus
pyruvate + NH3
-
-
-
?
4.3.1.17
L-serine
racemization reaction
661171
Mus musculus
D-serine
-
-
-
r
4.3.1.17
L-serine O-sulfate
elimination reaction
661171
Mus musculus
O-sulfopyruvate + NH3
-
-
-
?
4.3.1.17
L-threo-3-hydroxyaspartate
-
661171
Mus musculus
oxaloacetate + NH3
-
-
-
?
4.3.1.17
L-threonine
-
661171
Mus musculus
2-oxobutyrate + NH3
-
-
-
?
4.3.1.17
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
5.1.1.10
D-serine
-
661171
Mus musculus
L-serine
-
-
-
r
5.1.1.10
L-serine
-
661171
Mus musculus
D-serine
-
-
-
r
5.1.1.10
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
5.1.1.18
D-serine
-
661171
Mus musculus
pyruvate + NH3
-
-
-
?
5.1.1.18
D-serine
-
661171
Mus musculus
L-serine
-
-
-
r
5.1.1.18
L-serine
-
661171
Mus musculus
pyruvate + NH3
-
-
-
?
5.1.1.18
L-serine
-
661171
Mus musculus
D-serine
-
-
-
r
5.1.1.18
L-serine O-sulfate
-
661171
Mus musculus
O-sulfopyruvate + NH3
-
-
-
?
5.1.1.18
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.17
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.17
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
2.6
-
beta-chloro-L-alanine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
10.5
-
L-threonine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
31
-
L-threo-3-hydroxyaspartate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.10
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.1.1.18
0.043
-
L-erythro-3-hydroxyaspartate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.071
-
malonate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.55
-
Maleate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.64
-
L-cysteine-S-sulfate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.66
-
meso-tartrate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.69
-
Dihydroxyfumarate
pH 8.0, 37°C
Mus musculus
5.1.1.18
1.13
-
L-asparagine
pH 8.0, 37°C
Mus musculus
5.1.1.18
1.64
-
glycine
pH 8.0, 37°C
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.18
Dihydroxyfumarate
-
Mus musculus
5.1.1.18
glycine
competitive
Mus musculus
5.1.1.18
L-asparagine
-
Mus musculus
5.1.1.18
L-cysteine-S-sulfate
-
Mus musculus
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
Maleate
-
Mus musculus
5.1.1.18
malonate
-
Mus musculus
5.1.1.18
meso-tartrate
-
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.1.1.18
0.043
-
L-erythro-3-hydroxyaspartate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.071
-
malonate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.55
-
Maleate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.64
-
L-cysteine-S-sulfate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.66
-
meso-tartrate
pH 8.0, 37°C
Mus musculus
5.1.1.18
0.69
-
Dihydroxyfumarate
pH 8.0, 37°C
Mus musculus
5.1.1.18
1.13
-
L-asparagine
pH 8.0, 37°C
Mus musculus
5.1.1.18
1.64
-
glycine
pH 8.0, 37°C
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.17
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.17
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
4.3.1.17
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
36121
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
36123
-
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.17
beta-chloro-L-alanine
-
661171
Mus musculus
3-chloropropenoic acid + NH3
-
-
-
?
4.3.1.17
D-serine
elimination reaction
661171
Mus musculus
pyruvate + NH3
-
-
-
?
4.3.1.17
D-serine
racemization reaction
661171
Mus musculus
L-serine
-
-
-
r
4.3.1.17
L-serine
elimination reaction
661171
Mus musculus
pyruvate + NH3
-
-
-
?
4.3.1.17
L-serine
racemization reaction
661171
Mus musculus
D-serine
-
-
-
r
4.3.1.17
L-serine O-sulfate
elimination reaction
661171
Mus musculus
O-sulfopyruvate + NH3
-
-
-
?
4.3.1.17
L-threo-3-hydroxyaspartate
-
661171
Mus musculus
oxaloacetate + NH3
-
-
-
?
4.3.1.17
L-threonine
-
661171
Mus musculus
2-oxobutyrate + NH3
-
-
-
?
4.3.1.17
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
5.1.1.10
D-serine
-
661171
Mus musculus
L-serine
-
-
-
r
5.1.1.10
L-serine
-
661171
Mus musculus
D-serine
-
-
-
r
5.1.1.10
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
5.1.1.18
D-serine
-
661171
Mus musculus
pyruvate + NH3
-
-
-
?
5.1.1.18
D-serine
-
661171
Mus musculus
L-serine
-
-
-
r
5.1.1.18
L-serine
-
661171
Mus musculus
pyruvate + NH3
-
-
-
?
5.1.1.18
L-serine
-
661171
Mus musculus
D-serine
-
-
-
r
5.1.1.18
L-serine O-sulfate
-
661171
Mus musculus
O-sulfopyruvate + NH3
-
-
-
?
5.1.1.18
additional information
racemization and elimination activities reside at the same active site of enzyme. Racemization activity is specific to serine, elimination activity has a broader specificity for L-amino acids with a suitable leaving group at the beta-carbon
661171
Mus musculus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.17
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.10
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
5.1.1.18
?
x * 36121, MALDI-MS, x * 36123, calculated
Mus musculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.17
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
2.6
-
beta-chloro-L-alanine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
10.5
-
L-threonine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
4.3.1.17
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
4.3.1.17
31
-
L-threo-3-hydroxyaspartate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.10
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.10
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
0.49
-
L-serine O-sulfate
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.2
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus
5.1.1.18
4
-
L-serine
pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction
Mus musculus
5.1.1.18
14.5
-
D-serine
pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction
Mus musculus