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Literature summary extracted from
- Impact of the C-terminal domain of topoisomerase IIalpha on the DNA cleavage activity of the human enzyme (2005), Biochemistry, 44, 11546-11554.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.2.2 | expression of wild-type and truncation mutant Top2alphaDELTA1175 in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.6.2.2 | additional information | construction of a truncation mutant Top2alphaDELTA1175 cleaved at residue 1175, which shows 2-3fold increased DNA cleavage activity but reduced overall activity, and also a reduced response to anticancer drugs | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | amsacrine | - |
Homo sapiens |  |
| 5.6.2.2 | CP-115953 | - |
Homo sapiens | |
| 5.6.2.2 | EDTA | at 77 mM | Homo sapiens | |
| 5.6.2.2 | etoposide | - |
Homo sapiens | |
| 5.6.2.2 | additional information | the C-terminus of the enzyme is responsible for sensitivity to anticancer drugs | Homo sapiens | |
| 5.6.2.2 | SDS | 0.5% | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | KCl | - |
Homo sapiens | |
| 5.6.2.2 | Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.2 | dsDNA + ATP + H2O | Homo sapiens | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.2 | Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Homo sapiens | ? | - |
? | |
| 5.6.2.2 | kinetoplast DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA isolated from Crithidia fasciculata, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms | Homo sapiens | ? | - |
? | |
| 5.6.2.2 | additional information | the enzyme reaction is modulated by the C-terminus of the enzyme, which is also responsible for sensitivity to anti-cancer drugs | Homo sapiens | ? | - |
? | |
| 5.6.2.2 | negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.2.2 | More | coordination between subunits of wild-type and truncation mutant Top2alphaDELTA1175, overview | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.2 | Top2alpha | - |
Homo sapiens |
| 5.6.2.2 | topoisomerase IIalpha | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.2 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.2 | 7.9 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | ATP | dependent on | Homo sapiens | |
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Release 2023.1 (January 2023)
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