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Literature summary extracted from

  • Spencer, A.C.; Heck, A.; Takeuchi, N.; Watanabe, K.; Spremulli, L.L.
    Characterization of the human mitochondrial methionyl-tRNA synthetase (2004), Biochemistry, 43, 9743-9754.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.10 additional information bovine serum albumin has no effect on the enzyme Homo sapiens
6.1.1.10 spermine slight stimulation, additionally spermine increases the enzyme activity by stabilizing conformation of negatively charged DNA Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 mature enzyme form, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli Homo sapiens

General Stability

EC Number General Stability Organism
6.1.1.10 bovine serum albumin has no effect on the enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.00015
-
 tRNAMet pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme Homo sapiens
6.1.1.10 0.00016
-
 tRNAMet pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme Homo sapiens
6.1.1.10 0.0021
-
 tRNAMet pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme Homo sapiens
6.1.1.10 0.018
-
 L-methionine pH 8.2, 37°C, recombinant enzyme Homo sapiens
6.1.1.10 0.085
-
 ATP pH 8.2, 37°C, recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.10 mitochondrion mtMetRS Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 KCl stimulates Homo sapiens
6.1.1.10 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 67000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Homo sapiens
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Homo sapiens P56192 class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 recombinant His-tagged mature enzyme form from Escherichia coli by ion exchange chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.10 ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet two-step reaction process via anenzyme-L-Met-AMP intermediate Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Homo sapiens AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-methionine + tRNAMet human mitochondrial wild-type and mutant mtRNAs: T5048 deletion, and T5052C or T5012A point mutations, initiator tRNA from Escherichia coli, tRNAMet from Bos taurus Homo sapiens AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 additional information the enzyme performs ATP/diphosphate exchange in absence of substrates Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 monomer 1 * 67000, SDS-PAGE Homo sapiens
6.1.1.10 More domain structure, structure modeling based on crystal structures of Escherichia coli with PDB code 1QQR, overview, the enzyme contains the consensus motifs of class I aminoacyl-tRNA, but lacks the Zn2+ binding motif and the C-terminal dimerization region Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 methionyl-tRNA synthetase
-
 Homo sapiens
6.1.1.10 MetRS
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 0.019
-
 tRNAMet pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme Homo sapiens
6.1.1.10 0.021
-
 tRNAMet pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme Homo sapiens
6.1.1.10 0.033
-
 ATP pH 8.2, 37°C, recombinant enzyme Homo sapiens
6.1.1.10 0.41
-
 L-methionine pH 8.2, 37°C, recombinant enzyme Homo sapiens
6.1.1.10 11
-
 tRNAMet pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 7.8 8.2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Homo sapiens