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Literature summary extracted from

  • Brule, H.; Elliott, M.; Redlak, M.; Zehner, Z.E.; Holmes, W.M.
    Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein (2004), Biochemistry, 43, 9243-9255.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.228 glycerol TRM5 performs best between 25 and 30% glycerol, and is less active at lower concentrations. The enzyme retains 45% of its activity in the presence of 50% glycerol Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228
-
 Escherichia coli
2.1.1.228
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.228 DTT TrmD has maximal activity below 1 mM DTT and loses 20% of its activity above that value Escherichia coli
2.1.1.228 DTT TRM5 displays maximal activity above 1 mM DTT and loses 20% of its activity below that value Homo sapiens
2.1.1.228 glycerol the Escherichia coli TRmD performs best in the absence of glycerol, its activity decresaing linearly with increasing glycerol concentrations. No activity at 50% glycerol Escherichia coli
2.1.1.228 KCl the enzyme is most active in absence of KCl Escherichia coli
2.1.1.228 KCl TRM5 enzyme is stimulated 4fold by 100 mM KCl. TRM5 tends to lose all activity in 600 mM KCl Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.228 0.0000457
-
 guanine37 in yeast tRNAPhe possessing an A36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.0000546
-
 guanine37 in Escherichia coli tRNA1Leu pH 8.0, 37°C Homo sapiens
2.1.1.228 0.000075
-
 guanine37 in yeast tRNAAsp possessing a C36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.000244
-
 guanine37 in yeast tRNAAsp possessing a G36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.00058
-
 guanine37 in yeast tRNAAsp possessing an U36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.00059
-
 guanine37 in yeast tRNAAsp possessing an A36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.00123
-
 inosine37 in yeast tRNAAsp possessing a G36I37 sequence below 0.00123 pH 8.0, 37°C Homo sapiens
2.1.1.228 0.00546
-
 guanine37 in human mitochondrial tRNAPro pH 8.0, 37°C Homo sapiens
2.1.1.228 0.0057
-
 guanine37 in Escherichia coli tRNA1Leu pH 8.0, 37°C Escherichia coli
2.1.1.228 0.0086
-
 guanine37 in human mitochondrial tRNAPro pH 8.0, 37°C Escherichia coli
2.1.1.228 0.018
-
 guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence pH 8.0, 37°C Homo sapiens
2.1.1.228 0.019
-
 guanine37 in yeast tRNAAsp possessing a G36G37 sequence pH 8.0, 37°C Escherichia coli
2.1.1.228 0.046
-
 guanine37 in yeast tRNAAsp possessing an A36G37 sequence pH 8.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.228 KCl TRM5 enzyme is stimulated 4fold by 100 mM KCl. TRM5 tends to lose all activity in 600 mM KCl Homo sapiens
2.1.1.228 Mg2+ totally inactive without Mg2+ Escherichia coli
2.1.1.228 additional information no absolute requirement for Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.228 30586
-
 2 * 30586, calculated from sequence Escherichia coli
2.1.1.228 60500
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Homo sapiens
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Escherichia coli P0A873
-
-
2.1.1.228 Homo sapiens Q32P41
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228
-
 Escherichia coli
2.1.1.228
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 additional information guanosine37-methylation by TRM5 occurs regardless of the nature of the nucleotide at position 36. TRM5 also methylates inosine at position 37. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity. The enzyme does not methylate adenosine37, cytosine37 or uridine37 in tRNA. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity Homo sapiens ?
-
 ?
2.1.1.228 additional information TrmD recognizes the G36pG37 motif preferentially and does not methylate inosine. The TrmD enzyme is tolerant of alterations in tRNA-protein tertiary interactions as long as the core tRNA structure and the G36pG37 are present Escherichia coli ?
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNA(Asp) possessing a G36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing a G36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a C36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a C36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing an A36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an A36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an U36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an U36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe possessing an A36G37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe possessing an A36G37 sequence
-
 ?
2.1.1.228 S-adenosyl-L-methionine + inosine37 in yeast tRNAAsp possessing a G36I37 sequence
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methylinosine37 in yeast tRNAAsp possessing a G36I37 sequence
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.228 dimer 2 * 30586, calculated from sequence Escherichia coli
2.1.1.228 monomer 1 * 60500, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 TRM5
-
 Homo sapiens
2.1.1.228 TrmD
-
 Escherichia coli
2.1.1.228 tRNA-(N1G37) methyltransferase
-
 Escherichia coli
2.1.1.228 tRNA-(N1G37) methyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 37
-
 assay at Escherichia coli
2.1.1.228 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.228 7.4
-
-
 Escherichia coli
2.1.1.228 7.4
-
-
 Homo sapiens
2.1.1.228 8
-
 assay at Escherichia coli
2.1.1.228 8
-
 assay at Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.228 6.4 8.4 fairly active between pH 6.4 and pH 8.4 Escherichia coli
2.1.1.228 6.4 8.4 fairly active between pH 6.4 and pH 8.4 Homo sapiens