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Literature summary extracted from

  • Yong-Biao, J.; Islam, M.N.; Sueda, S.; Kondo, H.
    Identification of the catalytic residues involved in the carboxyl transfer of pyruvate carboxylase (2004), Biochemistry, 43, 5912-5920.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.1 expression in Escherichia coli JM109 Geobacillus thermodenitrificans

Protein Variants

EC Number Protein Variants Comment Organism
6.4.1.1 D543E mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 D649N mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 D713E mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 D713N mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 D762E mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 D762N mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 E576D mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 E576Q mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 E592Q mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 K712Q mutant with decreased activity Geobacillus thermodenitrificans
6.4.1.1 K712R mutant with decreased activity Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.28
-
 pyruvate D762N Geobacillus thermodenitrificans
6.4.1.1 0.29
-
 pyruvate D762E Geobacillus thermodenitrificans
6.4.1.1 0.3
-
 pyruvate wild type Geobacillus thermodenitrificans
6.4.1.1 0.41
-
 pyruvate K712R Geobacillus thermodenitrificans
6.4.1.1 0.44
-
 pyruvate E576D Geobacillus thermodenitrificans
6.4.1.1 0.49
-
 pyruvate E592Q Geobacillus thermodenitrificans
6.4.1.1 0.58
-
 pyruvate D713E Geobacillus thermodenitrificans
6.4.1.1 1.16
-
 pyruvate D713N Geobacillus thermodenitrificans
6.4.1.1 1.2
-
 pyruvate D649N Geobacillus thermodenitrificans
6.4.1.1 1.62
-
 pyruvate E576Q Geobacillus thermodenitrificans
6.4.1.1 16.2
-
 pyruvate D543E Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3- Geobacillus thermodenitrificans
-
 ADP + oxaloacetate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Geobacillus thermodenitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Geobacillus thermodenitrificans ADP + oxaloacetate + phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin
-
 Geobacillus thermodenitrificans