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Literature summary extracted from

  • Crepin, T.; Schmitt, E.; Blanquet, S.; Mechulam, Y.
    Three-dimensional structure of methionyl-tRNA synthetase from Pyrococcus abyssi (2004), Biochemistry, 43, 2635-2644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 modified gene metS, expression of M606 enzyme in Escherichia coli Pyrococcus abyssi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.10 purified recombinant M606 enzyme, hanging drop vapor diffusion method, from 0.1 M MES, pH 6.5, 3,5% PEG 6000, and 2% dioxane, 22°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.9 A resolution Pyrococcus abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 KCl
-
Pyrococcus abyssi
6.1.1.10 Mg2+
-
Pyrococcus abyssi
6.1.1.10 Zn2+ two Zn2+ per protomer, binding site structures Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Pyrococcus abyssi
-
AMP + diphosphate + L-methionyl-tRNAMet
-
r

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Pyrococcus abyssi Q9V011 class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 recombinant M606 enzyme from Escherichia coli by ion exchange chromatography and gel filtration Pyrococcus abyssi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.10 additional information
-
-
Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
Pyrococcus abyssi AMP + diphosphate + L-methionyl-tRNAMet
-
r
6.1.1.10 ATP + L-methionine + tRNAMet tRNA anticodon binding site structure Pyrococcus abyssi AMP + diphosphate + L-methionyl-tRNAMet
-
r

Subunits

EC Number Subunits Comment Organism
6.1.1.10 More three-dimensional structure, structure of the dimerization domain Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 methionyl-tRNA synthetase
-
Pyrococcus abyssi
6.1.1.10 MetRS
-
Pyrococcus abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 60
-
-
Pyrococcus abyssi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.10 42 60 the enzyme is nearly inactive at 25°C, highest activity at 60°C Pyrococcus abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 7.6
-
assay at Pyrococcus abyssi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
Pyrococcus abyssi