Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tse Sum Bui, B.; Lotierzo, M.; Escalettes, F.; Florentin, D.; Marquet, A.
    Further investigation on the turnover of Escherichia coli biotin synthase with dethiobiotin and 9-mercaptodethiobiotin as substrates (2004), Biochemistry, 43, 16432-16441.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.6 additional information not inhibitory: pyridoxal 5’-phosphate, deoxyadenosine Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.6 dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin reaction proceeds via intermediate involving the (2Fe-2S) cluster, with a rate-determining step following the formation of this intermediate Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 9-mercaptodethiobiotin + S-adenosyl-L-methionine presence of S-adenosyl-L-methionine is required Escherichia coli biotin + ?
-
 ?
2.8.1.6 dethiobiotin + sulfur
-
 Escherichia coli biotin
-
 ?
2.8.1.6 additional information reaction has a turnover of one. Enzyme has no cysteine desulfurase activity, the required sulfide has to be added as Na2S. The active enzyme cannot be fully restored after one turn Escherichia coli ?
-
 ?