Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xiang, J.; Jung, J.Y.; Sampson, N.S.
    Entropy effects on protein hinges: the reaction catalyzed by triosephosphate isomerase (2004), Biochemistry, 43, 11436-11445.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.1 K174G/T175G/A176G 16.5fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 7.9fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8fold decrease in turnover number for dihydroxyacetone phosphate, 2.6fold increase in Ki-value for arsenate, 10.4fold increase in Ki-value for 2-phosphoglycolate Gallus gallus
5.3.1.1 V167G/W168G 17.9fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 2.6fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8.6fold decrease in turnover number for dihydroxyacetone phosphate, 2.7fold increase in Ki-value for arsenate, 8.1fold increase in Ki-value for 2-phosphoglycolate Gallus gallus
5.3.1.1 V167G/W168G/K174G/T175G/A176G 2529fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 8.5fold increase in Km-value for D-glyceraldehyde 3-phosphate, 1720fold decrease in turnover number for dihydroxyacetone phosphate, 2.8fold increase in Ki-value for arsenate, 12.4fold increase in Ki-value for 2-phosphoglycolate Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 2-Phosphoglycolate
-
 Gallus gallus
5.3.1.1 arsenate
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.47
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
5.3.1.1 0.97
-
 dihydroxyacetone phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
5.3.1.1 1.2
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
5.3.1.1 3.7
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
5.3.1.1 4
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
5.3.1.1 11
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
5.3.1.1 15
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
5.3.1.1 18
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Gallus gallus dihydroxyacetone phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 0.25
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
5.3.1.1 1.7
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
5.3.1.1 50
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
5.3.1.1 54
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
5.3.1.1 240
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
5.3.1.1 261
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
5.3.1.1 430
-
 dihydroxyacetone phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
5.3.1.1 4300
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.1 11
-
 arsenate 30°C, pH 7.6, wild-type enzyme Gallus gallus
5.3.1.1 29
-
 arsenate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
5.3.1.1 30
-
 arsenate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
5.3.1.1 31
-
 arsenate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus