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Literature summary extracted from

  • Zhang, Q.; Hrmova, M.; Shirley, N.J.; Lahnstein, J.; Fincher, G.B.
    Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerases from barley (Hordeum vulgare L.) (2005), Biochem. J., 394, 115-124.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.2 NAD+ non-covalently bound, released from the enzyme after denaturation with aqueous ethanol. Each enzyme molecule contains one molecule of NAD+ Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 expression in Escherichia coli Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.2 UDP
-
 Hordeum vulgare
5.1.3.2 UMP
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 0.04
-
 UDP-Gal
-
 Hordeum vulgare
5.1.3.2 0.04
-
 UDP-galactose pH 8.0, 25°C Hordeum vulgare
5.1.3.2 0.055
-
 UDP-Glc
-
 Hordeum vulgare
5.1.3.2 0.055
-
 UDP-glucose pH 8.0, 25°C Hordeum vulgare
5.1.3.2 0.16
-
 UDP-GalNAc
-
 Hordeum vulgare
5.1.3.2 0.16
-
 UDP-GalNAc pH 8.0, 25°C Hordeum vulgare
5.1.3.2 0.2
-
 UDP-GlcNAc
-
 Hordeum vulgare
5.1.3.2 0.2
-
 UDP-GlcNAc pH 8.0, 25°C Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 39000
-
 each subunit Hordeum vulgare
5.1.3.2 39000
-
 x * 39000, recombinant enzyme, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Hordeum vulgare
-
 L.
-
5.1.3.2 Hordeum vulgare Q58IJ6
-
-
5.1.3.2 Hordeum vulgare L.
-
 L.
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.2 coleoptile levels of HvUGE1 mRNA are low in day 1 and day 3 coleoptiles, increases approximately 28fold in day 5 coleoptiles Hordeum vulgare
-
5.1.3.2 leaf expression of HvUGE1 is highest in leaf tips, rapidly decreases to very low levels in the basal region of the leaves Hordeum vulgare
-
5.1.3.2 root mRNA levels of HvUGE1 are very high in the maturation zone compared with other regions of the root Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-Gal
-
 Hordeum vulgare UDP-Glc
-
 r
5.1.3.2 UDP-Gal
-
 Hordeum vulgare L. UDP-Glc
-
 r
5.1.3.2 UDP-galactose
-
 Hordeum vulgare UDP-glucose
-
 r
5.1.3.2 UDP-GalNAc
-
 Hordeum vulgare UDP-GlcNAc
-
 ?
5.1.3.2 UDP-GalNAc
-
 Hordeum vulgare UDP-GlcNAc
-
 r
5.1.3.2 UDP-GalNAc
-
 Hordeum vulgare L. UDP-GlcNAc
-
 ?
5.1.3.2 UDP-Glc
-
 Hordeum vulgare UDP-Gal
-
 r
5.1.3.2 UDP-Glc
-
 Hordeum vulgare L. UDP-Gal
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.2 ? x * 39000, recombinant enzyme, SDS-PAGE Hordeum vulgare
5.1.3.2 dimer homodimer, 2 * 39000 Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 HvUGE1
-
 Hordeum vulgare
5.1.3.2 HvUGE2
-
 Hordeum vulgare
5.1.3.2 HvUGE3
-
 Hordeum vulgare
5.1.3.2 UDP-Gal 4-epimerase
-
 Hordeum vulgare
5.1.3.2 UDP-Glc 4-epimerase
-
 Hordeum vulgare
5.1.3.2 UGE
-
 Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.2 0.07
-
 UDP-GlcNAc 25°C, pH 8.0 Hordeum vulgare
5.1.3.2 0.12
-
 UDP-GalNAc 25°C, pH 8.0 Hordeum vulgare
5.1.3.2 11
-
 UDP-glucose 25°C, pH 8.0 Hordeum vulgare
5.1.3.2 21
-
 UDP-galactose 25°C, pH 8.0 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 8 9.5
-
 Hordeum vulgare
5.1.3.2 8 9.5 interconversion of UDP-glucose and UDP-galactose Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.2 NAD+ non-covalently bound, required in bacteria Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.3.2 0.032
-
 UMP
-
 Hordeum vulgare
5.1.3.2 0.032
-
 UMP 25°C, pH 8.0 Hordeum vulgare
5.1.3.2 0.036
-
 UDP
-
 Hordeum vulgare
5.1.3.2 0.036
-
 UDP 25°C, pH 8.0 Hordeum vulgare