BRENDA - Enzyme Database show

Regulation of cytosolic prostaglandin E synthase by phosphorylation

Kobayashi, T.; Nakatani, Y.; Tanioka, T.; Tsujimoto, M.; Nakajo, S.; Nakaya, K.; Murakami, M.; Kudo, I.; Biochem. J. 381, 59-69 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
A23187
-
Rattus norvegicus
5.3.99.3
bradykinin
-
Rattus norvegicus
5.3.99.3
Hsp90
maximal activity is achieved when the three components (cPGES, casein kinase II and Hsp90) are mixed at a molar proportion of 1:1:1
Rattus norvegicus
5.3.99.3
additional information
phosphorylation is crucial for the enzyme's function, phosphorylation by casein kinase II enhances cPGES activity 3fold
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
A151G
shows similar activity as the wild-type
Rattus norvegicus
5.3.99.3
S113A
fails to be activated by casein kinase II
Rattus norvegicus
5.3.99.3
S118A
fails to be activated by casein kinase II
Rattus norvegicus
5.3.99.3
Y14F
shows similar activity as the wild-type
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole
-
Rattus norvegicus
5.3.99.3
dexamethasone
anti-inflammatory glucocorticoid
Rattus norvegicus
5.3.99.3
geldanamycin
-
Rattus norvegicus
5.3.99.3
SB-203580
blocks the association of cPGES with casein kinase II and Hsp90, as well as cPGES phosphorylation
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0149
-
prostaglandin H2
cPGES plus casein kinase II plus Hsp90
Rattus norvegicus
5.3.99.3
0.0357
-
prostaglandin H2
cPGES plus casein kinase II
Rattus norvegicus
5.3.99.3
0.0666
-
prostaglandin H2
cPGES alone
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
cytosol
-
Rattus norvegicus
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
fibroblast
3Y1 cell
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.3
2
-
cPGES alone
Rattus norvegicus
5.3.99.3
2.2
-
cPGES plus casein kinase II
Rattus norvegicus
5.3.99.3
2.5
-
cPGES plus casein kinase II plus Hsp90
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
660973
Rattus norvegicus
Prostaglandin E2
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
A23187
-
Rattus norvegicus
5.3.99.3
bradykinin
-
Rattus norvegicus
5.3.99.3
Hsp90
maximal activity is achieved when the three components (cPGES, casein kinase II and Hsp90) are mixed at a molar proportion of 1:1:1
Rattus norvegicus
5.3.99.3
additional information
phosphorylation is crucial for the enzyme's function, phosphorylation by casein kinase II enhances cPGES activity 3fold
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
A151G
shows similar activity as the wild-type
Rattus norvegicus
5.3.99.3
S113A
fails to be activated by casein kinase II
Rattus norvegicus
5.3.99.3
S118A
fails to be activated by casein kinase II
Rattus norvegicus
5.3.99.3
Y14F
shows similar activity as the wild-type
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole
-
Rattus norvegicus
5.3.99.3
dexamethasone
anti-inflammatory glucocorticoid
Rattus norvegicus
5.3.99.3
geldanamycin
-
Rattus norvegicus
5.3.99.3
SB-203580
blocks the association of cPGES with casein kinase II and Hsp90, as well as cPGES phosphorylation
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0149
-
prostaglandin H2
cPGES plus casein kinase II plus Hsp90
Rattus norvegicus
5.3.99.3
0.0357
-
prostaglandin H2
cPGES plus casein kinase II
Rattus norvegicus
5.3.99.3
0.0666
-
prostaglandin H2
cPGES alone
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
cytosol
-
Rattus norvegicus
5829
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
fibroblast
3Y1 cell
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.3
2
-
cPGES alone
Rattus norvegicus
5.3.99.3
2.2
-
cPGES plus casein kinase II
Rattus norvegicus
5.3.99.3
2.5
-
cPGES plus casein kinase II plus Hsp90
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
660973
Rattus norvegicus
Prostaglandin E2
-
-
-
?