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Literature summary extracted from

  • Kobayashi, T.; Nakatani, Y.; Tanioka, T.; Tsujimoto, M.; Nakajo, S.; Nakaya, K.; Murakami, M.; Kudo, I.
    Regulation of cytosolic prostaglandin E synthase by phosphorylation (2004), Biochem. J., 381, 59-69.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.3 A23187
-
 Rattus norvegicus
5.3.99.3 bradykinin
-
 Rattus norvegicus
5.3.99.3 Hsp90 maximal activity is achieved when the three components (cPGES, casein kinase II and Hsp90) are mixed at a molar proportion of 1:1:1 Rattus norvegicus
5.3.99.3 additional information phosphorylation is crucial for the enzyme's function, phosphorylation by casein kinase II enhances cPGES activity 3fold Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expression in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.3 A151G shows similar activity as the wild-type Rattus norvegicus
5.3.99.3 S113A fails to be activated by casein kinase II Rattus norvegicus
5.3.99.3 S118A fails to be activated by casein kinase II Rattus norvegicus
5.3.99.3 Y14F shows similar activity as the wild-type Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.3 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole
-
 Rattus norvegicus
5.3.99.3 dexamethasone anti-inflammatory glucocorticoid Rattus norvegicus
5.3.99.3 geldanamycin
-
 Rattus norvegicus
5.3.99.3 SB-203580 blocks the association of cPGES with casein kinase II and Hsp90, as well as cPGES phosphorylation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.3 0.0149
-
 prostaglandin H2 cPGES plus casein kinase II plus Hsp90 Rattus norvegicus
5.3.99.3 0.0357
-
 prostaglandin H2 cPGES plus casein kinase II Rattus norvegicus
5.3.99.3 0.0666
-
 prostaglandin H2 cPGES alone Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 cytosol
-
 Rattus norvegicus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.99.3 fibroblast 3Y1 cell Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.99.3 2
-
 cPGES alone Rattus norvegicus
5.3.99.3 2.2
-
 cPGES plus casein kinase II Rattus norvegicus
5.3.99.3 2.5
-
 cPGES plus casein kinase II plus Hsp90 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin H2
-
 Rattus norvegicus Prostaglandin E2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 CPGES
-
 Rattus norvegicus
5.3.99.3 Prostaglandin E synthase
-
 Rattus norvegicus