Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Phin, S.; Kupferwasser, D.; Lam, J.; Lee-Fruman, K.K.
    Mutational analysis of ribosomal S6 kinase 2 shows differential regulation of its kinase activity from that of ribosomal S6 kinase 1 (2003), Biochem. J., 373, 583-591.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 additional information S6K2 is phosphorylated at T228, T338, and S370 leading to activation, T338 plays the key role Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 S370A site-directed mutagenesis, S6K2 mutant is inactive Mus musculus
2.7.11.1 S370D site-directed mutagenesis, S6K2 mutant is inactive Mus musculus
2.7.11.1 T228A site-directed mutagenesis, S6K2 mutant shows reduced activation compared to the wild-type enzyme Mus musculus
2.7.11.1 T228A/T338E site-directed mutagenesis, S6K2 mutant is inactive Mus musculus
2.7.11.1 T228E site-directed mutagenesis, S6K2 mutant shows highly reduced activation compared to the wild-type enzyme Mus musculus
2.7.11.1 T338E site-directed mutagenesis, S6K2 mutant is fully active and rapamycin-resistant, while the S6K1 mutant is inactive Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 additional information no inhibition of S6K2 by wortmannin or rapamycin, S6K2 phosphorylation by MEK of S410, S417, and S423 of the autoinhibition domain leads to inhibition of the enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 ribosome
-
 Mus musculus 5840
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + a protein Mus musculus
-
 ADP + a phosphoprotein
-
 ?
2.7.11.1 additional information Mus musculus mechanism of S6K2 regulation via phosphorylation and autoinhibition with T338 playing the key role, S6K2 is regulated in a mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase MEK-dependent manner, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mus musculus
-
 p70 ribosomal S6 kinase 1 S6K1 and its homologue ribosomal S6 kinase 2 S6K2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein phosphorylation of S6K2 is regulated via interdependent steps, overview, S6K2 is phosphorylated at T228 by PDK1 and at T338, playing the key role, and at S370, leading to activation, phosphorylation of S410, S417, and S423 of the autoinhibition domain by MEK leads to inhibition of the enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + a protein
-
 Mus musculus ADP + a phosphoprotein
-
 ?
2.7.11.1 additional information mechanism of S6K2 regulation via phosphorylation and autoinhibition with T338 playing the key role, S6K2 is regulated in a mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase MEK-dependent manner, overview Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 p70 ribosomal S6 kinase 1
-
 Mus musculus
2.7.11.1 ribosomal S6 kinase 2
-
 Mus musculus
2.7.11.1 S6K1
-
 Mus musculus
2.7.11.1 S6K2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.2
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Mus musculus