EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.151 | DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.151 | 0.00028 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.8, 30°C, 0.5 mM ATP, recombinant enzyme | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.151 | nucleus | predominantly, a basic cluster at the enzyme's C-terminus is involved in nuclear targeting | Homo sapiens | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.151 | KCl | - |
Homo sapiens | |
2.7.1.151 | Mg2+ | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.151 | 47219 | - |
x * 47219, amino acid sequence calculation | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.151 | Homo sapiens | Q8NFU5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.151 | recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.151 | ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | the catalytic site contains an important Ser-Ser-Leu-Leu motif | Homo sapiens | |
2.7.1.151 | ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | the catalytic site contains an important Ser-Ser-Leu-Leu motif | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.151 | 0.45 | - |
purified recombinant enzyme, 1D-myo-inositol 1,3,4,5,6-pentakisphosphate formation from 1D-myo-inositol 1,4,5-trisphosphate, 0.5 mM ATP | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.151 | 2 ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | 2 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
2.7.1.151 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
ir | |
2.7.1.151 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.151 | ? | x * 47219, amino acid sequence calculation | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.151 | inositol phosphate multikinase | - |
Homo sapiens |
2.7.1.151 | IPMK | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.151 | 30 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.151 | 7.8 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.151 | ATP | - |
Homo sapiens |