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Literature summary extracted from

  • Velasco-Garcia, R.; Gonzalez-Segura, L.; Munoz-Clares, R.A.
    Steady-state kinetic mechanism of the NADP+- and NAD+-dependent reactions catalysed by betaine aldehyde dehydrogenase from Pseudomonas aeruginosa (2000), Biochem. J., 352, 675-683.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 betaine product inhibition Pseudomonas aeruginosa
1.2.1.8 NADH product inhibition Pseudomonas aeruginosa
1.2.1.8 NADPH product inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.083
-
 NAD+ 30°C, pH 8.0 Pseudomonas aeruginosa
1.2.1.8 0.385
-
 NADP+ 30°C, pH 8.0 Pseudomonas aeruginosa
1.2.1.8 0.434
-
 Betaine aldehyde 30°C, pH 8.0, cosubstrate NADP+ Pseudomonas aeruginosa
1.2.1.8 0.506
-
 Betaine aldehyde 30°C, pH 8.0, cosubstrate NAD+ Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.8 betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ steady-state ordered mechanism, in which NAD+ binds first and NADH leaves last Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa betaine + NADH + H+
-
 ?
1.2.1.8 betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa betaine + NADPH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.8 261
-
 NAD+ 30°C, pH 8.0 Pseudomonas aeruginosa
1.2.1.8 276
-
 NADP+ 30°C, pH 8.0 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Pseudomonas aeruginosa
1.2.1.8 NADP+
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.8 0.047
-
 NADH 30°C, pH 8.0 Pseudomonas aeruginosa
1.2.1.8 0.185
-
 NADPH 30°C, pH 8.0 Pseudomonas aeruginosa
1.2.1.8 0.209
-
 betaine 30°C, pH 8.0, cosubstrate NADP+ Pseudomonas aeruginosa
1.2.1.8 0.289
-
 betaine 30°C, pH 8.0, cosubstrate NAD+ Pseudomonas aeruginosa