Literature summary extracted from

Baldwin, J.E.; Adlington, R.M.; Coates, J.B.; Crabbe, M.J.; Crouch, N.P.; Keeping, J.W.; Knight, G.C.; Schofield, C.J.; Ting, H.H.; Vallejo, C.A.; et al.
Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities (1987), Biochem. J., 245, 831-841.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.14.11.26	ammonium chloride	-	Acremonium chrysogenum
1.14.11.26	ammonium sulfate	-	Acremonium chrysogenum
1.14.11.26	ascorbate	required	Acremonium chrysogenum
1.14.11.26	dithiothreitol	up to 30% activation in presence of dithiothreitol	Acremonium chrysogenum
1.14.20.1	ammonium chloride	-	Acremonium chrysogenum
1.14.20.1	ammonium sulfate	-	Acremonium chrysogenum
1.14.20.1	ascorbate	required	Acremonium chrysogenum
1.14.20.1	dithiothreitol	up to 30% activation in presence of dithiothreitol	Acremonium chrysogenum

General Stability

EC Number	General Stability	Organism
1.14.11.26	sodium phosphate and Bis/Tris buffers are inhibitory	Acremonium chrysogenum
1.14.20.1	sodium phosphate and Bis/Tris buffers are inhibitory	Acremonium chrysogenum

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.11.26	(2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate	reversible, 0.04 mM, 90% inhibition	Acremonium chrysogenum
1.14.11.26	ammonium hydrogencarbonate	100-500 mM, complete inactivation	Acremonium chrysogenum
1.14.11.26	bathophenanthroline	-	Acremonium chrysogenum
1.14.11.26	N-ethylmaleimide	-	Acremonium chrysogenum
1.14.20.1	(2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate	reversible, 0.04 mM, 90% inhibition	Acremonium chrysogenum
1.14.20.1	ammonium hydrogencarbonate	100-500 mM, complete inactivation	Acremonium chrysogenum
1.14.20.1	bathophenanthroline	-	Acremonium chrysogenum
1.14.20.1	N-ethylmaleimide	-	Acremonium chrysogenum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.11.26	40000	-	x * 40000, SDS-PAGE	Acremonium chrysogenum
1.14.20.1	40000	-	x * 40000, SDS-PAGE	Acremonium chrysogenum

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.11.26	Acremonium chrysogenum	-	enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26	-
1.14.20.1	Acremonium chrysogenum	-	enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.11.26	-	Acremonium chrysogenum
1.14.20.1	-	Acremonium chrysogenum

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.14.20.1	38.5	-	pH 7.4, 27°C	Acremonium chrysogenum

Storage Stability

EC Number	Storage Stability	Organism
1.14.11.26	-70°C, presence of dithiothreitol, stable for 4 weeks	Acremonium chrysogenum
1.14.11.26	4°C, presence of dithiothreitol, half-life is 48 h	Acremonium chrysogenum
1.14.20.1	-70°C, presence of dithiothreitol, stable for 4 weeks	Acremonium chrysogenum
1.14.20.1	4°C, presence of dithiothreitol, half-life is 48 h	Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.11.26	deacetoxycephalosporin C + 2-oxoglutarate + O2	-	Acremonium chrysogenum	deacetylcephalosporin C + succinate + CO2 + H2O	-	?
1.14.20.1	additional information	no substrate: pyruvate, 2-oxoadipate	Acremonium chrysogenum	?	-	?
1.14.20.1	penicillin N + 2-oxoglutarate + O2	-	Acremonium chrysogenum	deacetoxycephalosporin C + succinate + CO2 + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.11.26	?	x * 40000, SDS-PAGE	Acremonium chrysogenum
1.14.20.1	?	x * 40000, SDS-PAGE	Acremonium chrysogenum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.14.11.26	7.5	-	-	Acremonium chrysogenum
1.14.20.1	7.5	-	-	Acremonium chrysogenum

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Release 2023.1 (January 2023)