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Literature summary extracted from

  • Allan, B.; Kropinski, A.M.
    DNA-dependent RNA polymerase from Pseudomonas aeruginosa (1987), Biochem. Cell Biol., 65, 776-782.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.6 each freeze-thaw cycle results in significant loss of activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 actinomycin D
-
 Pseudomonas aeruginosa
2.7.7.6 heparin
-
 Pseudomonas aeruginosa
2.7.7.6 KCl above 10 mM Pseudomonas aeruginosa
2.7.7.6 rifampicin
-
 Pseudomonas aeruginosa
2.7.7.6 Streptolydigin
-
 Pseudomonas aeruginosa
2.7.7.6 streptovaracin
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ required, optimal activity at 10 mM MgCl2 Pseudomonas aeruginosa
2.7.7.6 Mn2+ can partially replace Mg2+, 20% of the activity with Mg2+ Pseudomonas aeruginosa
2.7.7.6 additional information KCl, ZnCl2 and CaCl2 can not replace MgCl2 in the assay mixture Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 45000
-
 beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE Pseudomonas aeruginosa
2.7.7.6 87000
-
 beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE Pseudomonas aeruginosa
2.7.7.6 148000
-
 beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE Pseudomonas aeruginosa
2.7.7.6 157000
-
 beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.000178
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.7.7.6 4°C, -20°C or -70°C, 50% w/v glycerol, stable for more than 3 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme is able to use a variety of DNA templates. DNA from bacteriphage phiPLS27 is transcribed more efficiently than DNA isolated from lamda or herring sperm. DNA isolated from bacteriophage T7 and T7 D111 is utilized more efficiently Pseudomonas aeruginosa diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 oligomer beta',beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 8.5
-
 Tris-HCl buffer Pseudomonas aeruginosa
2.7.7.6 9
-
 glycine-NaOH buffer Pseudomonas aeruginosa