EC Number | Application | Comment | Organism |
---|---|---|---|
3.3.2.10 | medicine | kinetic analysis of the effects of human enzyme polymorphisms on the N-terminal phosphatase activity of soluble epoxide hydrolase activity | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.76 | expression of enzyme in insect cells using the baculovirus infection system | Rattus norvegicus |
3.1.3.76 | expression of His-tagged wild-type and mutant enzymes in insect cells using the baculovirus infection system | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.76 | C154Y | the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme | Homo sapiens |
3.1.3.76 | R103C | the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme | Homo sapiens |
3.1.3.76 | R103C/R287Q | the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme | Homo sapiens |
3.1.3.76 | R287Q | the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.76 | additional information | no inhibition of the phosphatase activity by 1-cyclohexyl-3-dodecyl urea at 0.1 mM | Homo sapiens | |
3.1.3.76 | orthovanadate | 65% inhibition of the phosphatase activity at 0.1 mM | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.76 | 0.024 | - |
farnesyl phosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.033 | - |
geranylgeranyl diphosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.084 | - |
farnesyl diphosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.124 | - |
geranyl phosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 1.746 | - |
geranylgeranyl phosphate | recombinant enzyme | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.76 | cytosol | - |
Homo sapiens | 5829 | - |
3.1.3.76 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.76 | Mg2+ | dependent on | Rattus norvegicus | |
3.1.3.76 | Mg2+ | dependent on, maximal activity at 0.1 mM | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.76 | Homo sapiens | - |
- |
- |
3.1.3.76 | Rattus norvegicus | - |
- |
- |
3.3.2.10 | Homo sapiens | - |
bifunctional epoxide hydrolase/phosphatase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.76 | recombinant enzyme by S-benzyl affinity chromatography with 4-fluorochalcone oxide as eluent | Rattus norvegicus |
3.1.3.76 | recombinant His-tagged wild-type and mutant enzymes from insect cells by nickel affinity chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.3.76 | (9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Homo sapiens | |
3.1.3.76 | (9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.76 | liver | - |
Homo sapiens | - |
3.1.3.76 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.76 | 0.03 | - |
purified recombinant enzyme, substrate isopentenyl phosphate | Homo sapiens |
3.1.3.76 | 0.25 | - |
purified recombinant enzyme, substrate geranyl phosphate | Homo sapiens |
3.1.3.76 | 0.49 | - |
purified recombinant enzyme, substrate farnesyl phosphate | Homo sapiens |
3.1.3.76 | 0.53 | - |
purified recombinant enzyme, substrate geranylgeranyl phosphate | Homo sapiens |
3.1.3.76 | 0.8 | - |
purified recombinant enzyme, substrate geranyl phosphate | Rattus norvegicus |
3.1.3.76 | 0.85 | - |
purified recombinant enzyme, substrate isopentenyl phosphate | Rattus norvegicus |
3.1.3.76 | 0.99 | - |
purified recombinant enzyme, substrate farnesyl phosphate | Rattus norvegicus |
3.1.3.76 | 1.05 | - |
purified recombinant enzyme, substrate geranylgeranyl phosphate | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.76 | farnesyl diphosphate + H2O | - |
Homo sapiens | farnesol + phosphate | - |
? | |
3.1.3.76 | farnesyl phosphate + H2O | - |
Homo sapiens | farnesol + phosphate | - |
? | |
3.1.3.76 | farnesyl phosphate + H2O | - |
Rattus norvegicus | farnesol + phosphate | - |
? | |
3.1.3.76 | geranyl diphosphate + H2O | - |
Homo sapiens | geraniol + phosphate | - |
? | |
3.1.3.76 | geranyl phosphate + H2O | - |
Homo sapiens | geraniol + phosphate | - |
? | |
3.1.3.76 | geranyl phosphate + H2O | - |
Rattus norvegicus | geraniol + phosphate | - |
? | |
3.1.3.76 | geranylgeranyl diphosphate + H2O | - |
Homo sapiens | geranylgeraniol + phosphate | - |
? | |
3.1.3.76 | geranylgeranyl phosphate + H2O | - |
Homo sapiens | geranylgeraniol + phosphate | - |
? | |
3.1.3.76 | geranylgeranyl phosphate + H2O | - |
Rattus norvegicus | geranylgeraniol + phosphate | - |
? | |
3.1.3.76 | isopentenyl diphosphate + H2O | - |
Homo sapiens | isopentenol + phosphate | - |
? | |
3.1.3.76 | isopentenyl phosphate + H2O | - |
Homo sapiens | isopentenol + phosphate | - |
? | |
3.1.3.76 | isopentenyl phosphate + H2O | - |
Rattus norvegicus | isopentenol + phosphate | - |
? | |
3.3.2.10 | additional information | kinetic analysis of the effects of human enzyme polymorphisms on the N-terminal phosphatase activity of soluble epoxide hydrolase activity | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.76 | More | c.f. EC 3.3.2.10 | Homo sapiens |
3.1.3.76 | More | c.f. EC 3.3.2.10 | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.76 | 0.13 | - |
geranylgeranyl diphosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.25 | - |
farnesyl diphosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.51 | - |
geranyl phosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 0.71 | - |
farnesyl phosphate | recombinant enzyme | Homo sapiens | |
3.1.3.76 | 1.85 | - |
geranylgeranyl phosphate | recombinant enzyme | Homo sapiens |