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Literature summary extracted from
- Enzymatic and structural characterization of type II isopentenyl diphosphate isomerase from hyperthermophilic archaeon Thermococcus kodakaraensis (2005), Biochem. Biophys. Res. Commun., 331, 1127-1136.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.3.2 | expression in Escherichia coli strain BL21(DE3) | Thermococcus kodakarensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.2 | 0.046 | - |
isopentenyl diphosphate | pH 8.0, 50°C | Thermococcus kodakarensis |  |
| 5.3.3.2 | 0.047 | - |
isopentenyl diphosphate | pH 8.0, 55°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.063 | - |
isopentenyl diphosphate | pH 8.0, 60°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.065 | - |
isopentenyl diphosphate | pH 8.0, 70°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.071 | - |
isopentenyl diphosphate | pH 8.0, 65°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.077 | - |
isopentenyl diphosphate | pH 8.0, 75°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.084 | - |
isopentenyl diphosphate | pH 8.0, 80°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.093 | - |
isopentenyl diphosphate | pH 8.0, 90°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.187 | - |
isopentenyl diphosphate | pH 8.0, 82.5°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.199 | - |
isopentenyl diphosphate | pH 8.0, 87.5°C | Thermococcus kodakarensis | |
| 5.3.3.2 | 0.211 | - |
isopentenyl diphosphate | pH 8.0, 85°C | Thermococcus kodakarensis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.2 | Mg2+ | - |
Thermococcus kodakarensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.2 | 41492 | - |
8 * 41492, calculated | Thermococcus kodakarensis |
| 5.3.3.2 | 345000 | - |
gel filtration chromatography | Thermococcus kodakarensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.2 | Thermococcus kodakarensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.2 | - |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.2 | isopentenyl diphosphate | - |
Thermococcus kodakarensis | dimethylallyl diphosphate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.3.2 | octamer | 8 * 41492, calculated | Thermococcus kodakarensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.3.2 | IDI | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.2 | 80 | - |
maximum activity | Thermococcus kodakarensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.2 | 50 | 90 | - |
Thermococcus kodakarensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.2 | 70 | - |
1h, IDI retained 50% of the activity | Thermococcus kodakarensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.2 | FMN | - |
Thermococcus kodakarensis | |
| 5.3.3.2 | NADPH | - |
Thermococcus kodakarensis | |
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