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Literature summary extracted from

  • Ronimus, R.S.; Morgan, H.W.
    Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase (2004), Biochem. Biophys. Res. Commun., 315, 652-658.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.147 additional information no activation by D-glucose 6-phosphate, no or nearly no inhibition by L-glucose, glucose 1-phosphate, fructose 1-phosphate, 2,3-bisphosphoglycerate, succinate, proline, N-acetylglucosamine, diphosphate, mannose, galactose, fructose, ribose, NH4Cl, IMP, glucosamine, inositol, and phosphocreatine Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.147 DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.147 Mg2+ excess Mg2+ is inhibitory, 55% inhibition at 55 mM, 41% at 15 mM, and 22% at 10 mM Mus musculus
2.7.1.147 additional information no inhibition by D-glucose 6-phosphate, no or nearly no inhibition by L-glucose, glucose 1-phosphate, fructose 1-phosphate, 2,3-bisphosphoglycerate, succinate, proline, N-acetylglucosamine, diphosphate, mannose, galactose, fructose, ribose, NH4Cl, IMP, glucosamine, inositol, phosphocreatine, ATP, GTP, CTP, GDP, UTP, TTP, NADP+, oxaloacetate, TDP, CDP, UDP, palmitate, D-glucose 1,6-bisphosphate, and several other compounds, overview Mus musculus
2.7.1.147 NaCl 84% of maximal activity at 0.5 M, 48% of maximal activity at 1 M Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 additional information
-
additional information
-
Mus musculus
2.7.1.147 0.028
-
ADP at 0.8 mM and an ADP:Mg2+ ratio of 1:1, pH 7.0, 37°C Mus musculus
2.7.1.147 0.096
-
D-glucose at below 0.35 mM, pH 7.0, 37°C Mus musculus
2.7.1.147 20
-
GDP pH 7.0, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.147 KCl required at 0.5 M for full enzyme activity Mus musculus
2.7.1.147 Mg2+ best at ADP-Mg2+ ratio of 1:1, excess Mg2+ is inhibitory Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.147 51500
-
1 * 51500, recombinant enzyme, SDS-PAGE Mus musculus
2.7.1.147 60300
-
recombinant enzyme, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.147 ADP + D-glucose Mus musculus
-
AMP + D-glucose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Mus musculus Q8VDL4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147 recombinant enzyme from Escherichia coli strain DH5alpha by nickel affinity and ion exchange chromatography, to homogeneity Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.147 11.7
-
purified recombinant enzyme, pH 7.0, 37°C, substrate D-glucose Mus musculus
2.7.1.147 17
-
purified recombinant enzyme, pH 6.0, 37°C, substrate D-glucose Mus musculus
2.7.1.147 29.3
-
purified recombinant enzyme, pH 9.0, 37°C, substrate D-glucose Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-fructose 10% of the activity with D-glucose Mus musculus AMP + D-fructose 6-phosphate
-
?
2.7.1.147 ADP + D-glucose
-
Mus musculus AMP + D-glucose 6-phosphate
-
?
2.7.1.147 ADP + D-glucose best substrate concentration is 0.35 mM glucose, high concentrations are inhibitory Mus musculus AMP + D-glucose 6-phosphate
-
?
2.7.1.147 ADP + D-mannose 20% of the activity with D-glucose Mus musculus AMP + D-mannose 6-phosphate
-
?
2.7.1.147 CDP + D-glucose 12% of the activity with ADP Mus musculus CMP + D-glucose 6-phosphate
-
?
2.7.1.147 GDP + D-glucose 55% of the activity with ADP Mus musculus GMP + D-glucose 6-phosphate
-
?
2.7.1.147 additional information no activity with ATP, GTP, CTP, UTP, TTP, diphosphate, polyphosphate, TDP, UDP, acetyl-phosphate, phosphoarginine, carbamoyl phosphate, phosphocreatine, and phosphoenolpyruvate as phosphoryl donors Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.147 monomer 1 * 51500, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 ADP-dependent glucokinase
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.147 37
-
assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.147 5.8 6.5 bimodal optima at pH 5.8-6.5 and at 8.8-9.0 Mus musculus
2.7.1.147 8.8 9 bimodal optima at pH 5.8-6.5 and at 8.8-9.0 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.147 6 9
-
Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.147 ADP ADP-Mg2+ ratio of 1:1 Mus musculus