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Literature summary extracted from

  • Bräsen, C.; Schönheit, P.
    Unusual ADP-forming acetyl-coenzyme A synthetases from the mesophilic halophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (2004), Arch. Microbiol., 182, 277-287.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.13 expression in Escherichia coli Haloarcula marismortui
6.2.1.13 expression in Escherichia coli Pyrobaculum aerophilum

General Stability

EC Number General Stability Organism
6.2.1.13 requires high salt concentrations for long-term stability Haloarcula marismortui

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.13 Isobutyrate 10 mM, complete inhibition Haloarcula marismortui
6.2.1.13 NaCl 2-3 M, 40% loss of activity Haloarcula marismortui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.04
-
 isobutyryl-CoA pH 6.5, 85°C Pyrobaculum aerophilum
6.2.1.13 0.046
-
 ADP pH 7.5, 37°C Haloarcula marismortui
6.2.1.13 0.08
-
 CoA pH 7.5, 37°C Haloarcula marismortui
6.2.1.13 0.11
-
 phenylacetyl-CoA pH 6.5, 85°C Pyrobaculum aerophilum
6.2.1.13 0.12
-
 acetyl-CoA pH 6.5, 85°C Pyrobaculum aerophilum
6.2.1.13 0.3
-
 ATP pH 7.5, 37°C Haloarcula marismortui
6.2.1.13 0.45
-
 acetyl-CoA pH 7.5, 37°C Haloarcula marismortui
6.2.1.13 1.1
-
 acetate pH 7.5, 37°C Haloarcula marismortui
6.2.1.13 2
-
 phosphate pH 7.5, 37°C Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.13 Mg2+ optimal MgCl2 concentration: 30 mM in absence of KCl, 20 mM in presence of 3 M KCl Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.13 87000
-
 2 * 87000, SDS-PAGE Haloarcula marismortui
6.2.1.13 166000
-
 gel filtration Haloarcula marismortui

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA Haloarcula marismortui
-
 ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + acetyl-CoA Pyrobaculum aerophilum
-
 ATP + acetate + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Haloarcula marismortui
-
-
-
6.2.1.13 Pyrobaculum aerophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13
-
 Haloarcula marismortui
6.2.1.13
-
 Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.13 additional information
-
-
 Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA
-
 Haloarcula marismortui ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + acetyl-CoA
-
 Pyrobaculum aerophilum ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + isobutyryl-CoA
-
 Pyrobaculum aerophilum ATP + isobutyrate + CoA
-
 r
6.2.1.13 ADP + phosphate + phenylacetyl-CoA
-
 Pyrobaculum aerophilum ATP + phenylacetate + CoA
-
 r
6.2.1.13 ATP + acetate + CoA
-
 Haloarcula marismortui ADP + phosphate + acetyl-CoA
-
 r
6.2.1.13 ATP + butyrate + CoA at 98% of the activity with acetate Haloarcula marismortui ADP + phosphate + butyryl-CoA
-
 ?
6.2.1.13 ATP + isobutyrate + CoA at 38% of the activity with acetate Haloarcula marismortui ADP + phosphate + isobutyryl-CoA
-
 ?
6.2.1.13 ATP + isovalerate + CoA at 25% of the activity with acetate Haloarcula marismortui ADP + phosphate + isovaleryl-CoA
-
 ?
6.2.1.13 ATP + propionate + CoA at 108% of the activity with acetate Haloarcula marismortui ADP + phosphate + propionyl-CoA
-
 ?
6.2.1.13 GTP + acetate + CoA 11% of the activity with ATP Haloarcula marismortui GDP + phosphate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.13 dimer 2 * 87000, SDS-PAGE Haloarcula marismortui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.13 43
-
-
 Haloarcula marismortui
6.2.1.13 93
-
 above Pyrobaculum aerophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.13 30 50 30°C: about 50% of maximal activity, 50°C: about 80% of maximal activity Haloarcula marismortui

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.13 80
-
 120 min, no significant loss of activity Pyrobaculum aerophilum
6.2.1.13 90
-
 half-life: 105 min Pyrobaculum aerophilum
6.2.1.13 100
-
 half-life: 13 min. Completely stable for 120 min in presence of 1 M (NH4)2SO4 Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.13 6.5
-
-
 Pyrobaculum aerophilum
6.2.1.13 7.5
-
-
 Haloarcula marismortui

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.13 5.5 7.5 pH 5.5: about 40% of maximal activity, pH 7.5: about 20% of maximal activity Pyrobaculum aerophilum
6.2.1.13 6.5 8.5 pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity Haloarcula marismortui