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Literature summary extracted from

  • Ruan, K.H.; Deng, H.; Wu, J.; So, S.P.
    The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase (2005), Arch. Biochem. Biophys., 435, 372-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.4 expression in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.4 DELTA-20-PGIS PGH2 presentation to the membrane-bound mutant has a 2 to 3fold delay compared to that of membrane-bound wild-type PGIS Homo sapiens
5.3.99.4 PGIS/TXAS(8-27) the first 20 membrane anchor residues of human PGIS are replaced by the corresponding membrane anchor region of human TXAS (thromboxane A2 synthase). Efficiency of the isomerization of PGH2 to PGI2 is slowed down with approximately 25, 65, and 85% at 30, 60, and 120 s reactions, compared to the wild-type PGIS, respectively Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.4 membrane membrane-bound Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.99.4 57000
-
 chimer PGIS/TXAS(8-27), monomer, SDS-PAGE Homo sapiens
5.3.99.4 57000
-
 mutant delta-20-PGIS, monomer, SDS-PAGE Homo sapiens
5.3.99.4 57000
-
 wild-type, monomer, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.4 Prostaglandin H2 Homo sapiens in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2 Prostaglandin I2
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
5.3.99.4 Triton X-100 within 30 s, about 75% of PGH2 is presented to the PGIS active side and isomerized to PGI2 by the membrane-bound PGIS. In contrast, only about 20% of the substrate is isomerized to the product by the detergent-solubilized (Triton X-100) PGIS. Replacement of the membrane with detergent does not alter the PGIS active side conformation, but affects the substrate presentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.4 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.4 Prostaglandin H2
-
 Homo sapiens Prostaglandin I2
-
 ?
5.3.99.4 Prostaglandin H2 in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2 Homo sapiens Prostaglandin I2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.4 PGIS
-
 Homo sapiens
5.3.99.4 prostacyclin synthase
-
 Homo sapiens
5.3.99.4 Prostaglandin I2 synthase
-
 Homo sapiens