BRENDA - Enzyme Database show

The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase

Ruan, K.H.; Deng, H.; Wu, J.; So, S.P.; Arch. Biochem. Biophys. 435, 372-381 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.4
expression in COS-7 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.4
DELTA-20-PGIS
PGH2 presentation to the membrane-bound mutant has a 2 to 3fold delay compared to that of membrane-bound wild-type PGIS
Homo sapiens
5.3.99.4
PGIS/TXAS(8-27)
the first 20 membrane anchor residues of human PGIS are replaced by the corresponding membrane anchor region of human TXAS (thromboxane A2 synthase). Efficiency of the isomerization of PGH2 to PGI2 is slowed down with approximately 25, 65, and 85% at 30, 60, and 120 s reactions, compared to the wild-type PGIS, respectively
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
membrane
membrane-bound
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
57000
-
chimer PGIS/TXAS(8-27), monomer, SDS-PAGE; mutant delta-20-PGIS, monomer, SDS-PAGE; wild-type, monomer, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Homo sapiens
in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2
Prostaglandin I2
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
5.3.99.4
Triton X-100
within 30 s, about 75% of PGH2 is presented to the PGIS active side and isomerized to PGI2 by the membrane-bound PGIS. In contrast, only about 20% of the substrate is isomerized to the product by the detergent-solubilized (Triton X-100) PGIS. Replacement of the membrane with detergent does not alter the PGIS active side conformation, but affects the substrate presentation
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.4
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
Prostaglandin H2
-
660808
Homo sapiens
Prostaglandin I2
-
-
-
?
5.3.99.4
Prostaglandin H2
in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2
660808
Homo sapiens
Prostaglandin I2
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
expression in COS-7 cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.4
DELTA-20-PGIS
PGH2 presentation to the membrane-bound mutant has a 2 to 3fold delay compared to that of membrane-bound wild-type PGIS
Homo sapiens
5.3.99.4
PGIS/TXAS(8-27)
the first 20 membrane anchor residues of human PGIS are replaced by the corresponding membrane anchor region of human TXAS (thromboxane A2 synthase). Efficiency of the isomerization of PGH2 to PGI2 is slowed down with approximately 25, 65, and 85% at 30, 60, and 120 s reactions, compared to the wild-type PGIS, respectively
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
membrane
membrane-bound
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
57000
-
chimer PGIS/TXAS(8-27), monomer, SDS-PAGE; mutant delta-20-PGIS, monomer, SDS-PAGE; wild-type, monomer, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
Prostaglandin H2
Homo sapiens
in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2
Prostaglandin I2
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
5.3.99.4
Triton X-100
within 30 s, about 75% of PGH2 is presented to the PGIS active side and isomerized to PGI2 by the membrane-bound PGIS. In contrast, only about 20% of the substrate is isomerized to the product by the detergent-solubilized (Triton X-100) PGIS. Replacement of the membrane with detergent does not alter the PGIS active side conformation, but affects the substrate presentation
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
Prostaglandin H2
-
660808
Homo sapiens
Prostaglandin I2
-
-
-
?
5.3.99.4
Prostaglandin H2
in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2
660808
Homo sapiens
Prostaglandin I2
-
-
-
?