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Literature summary extracted from

  • Takakura, T.; Ito, T.; Yagi, S.; Notsu, Y.; Itakura, T.; Nakamura, T.; Inagaki, K.; Esaki, N.; Hoffman, R.M.; Takimoto, A.
    High-level expression and bulk crystallization of recombinant L-methionine gamma-lyase, an anticancer agent (2006), Appl. Microbiol. Biotechnol., 70, 183-192.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.11 drug development endotoxins cause fever in humans and must be eliminated from biopharmaceuticals intended for parenteral administration which are produced by Escherichia coli. Endotoxins can be drastically removed by anion-exchange column chromatography. Thus, the combination of crystallization and anion-exchange column chromatography is suitable for purification of the enzyme Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.11
-
 Pseudomonas putida

General Stability

EC Number General Stability Organism
4.4.1.11 the purified enzyme can be stored at -80°C for several months with no significant loss of activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 42000
-
 4 * 42000, SDS-PAGE, gel filtration Pseudomonas putida
4.4.1.11 160000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Pseudomonas putida
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 54
-
 crystals Pseudomonas putida
4.4.1.11 57
-
 purified recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.11 tetramer 4 * 42000, SDS-PAGE, gel filtration Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 L-methionine gamma-lyase
-
 Pseudomonas putida
4.4.1.11 METase
-
 Pseudomonas putida