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Literature summary extracted from
- Effect of Sorghum vulgare phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase coexpression on succinate production in mutant strains of Escherichia coli (2005), Appl. Microbiol. Biotechnol., 67, 515-523.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.1.31 | biotechnology | phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase are overexpressed in Escherichia coli concurrently to improve the production of succinate. This coexpression system is also applied to mutant strains of Escherichia coli strategically designed by inactivating the competing pathways of succinate formation | Sorghum bicolor |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.31 | phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase are overexpressed in Escherichia coli concurrently to improve the production of succinate. This coexpression system is also applied to mutant strains of Escherichia coli strategically designed by inactivating the competing pathways of succinate formation. Coexpression of phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase is effective in depleting pyruvate accumulation and increasing the production of metabolites | Sorghum bicolor |
| 6.4.1.1 | expression in Escherichia coli wild-type GJT001 and mutants YBS121 | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.1 | ATP + pyruvate + HCO3- | Lactococcus lactis | - |
ADP + oxaloacetate + phosphate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.31 | Sorghum bicolor | - |
- |
- |
| 6.4.1.1 | Lactococcus lactis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.1 | ATP + pyruvate + HCO3- | - |
Lactococcus lactis | ADP + oxaloacetate + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.4.1.1 | PYC | - |
Lactococcus lactis |
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