Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Park, J.H.; Batt, C.A.
    Restoration of a defective Lactococcus lactis xylose isomerase (2004), Appl. Environ. Microbiol., 70, 4318-4325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in Escherichia coli Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 E407K 2% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 K407E 92% of wild-type activity Lactococcus lactis
5.3.1.5 R202M 9% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 R202M/V275A 26% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 R202M/Y218D 9% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 R202M/Y218D/V275A 62% of strain IO-1 wild-type activity and soluble Lactococcus lactis
5.3.1.5 S247A 97% of wild-type activity Lactococcus lactis
5.3.1.5 S247A/K407E 79% of wild-type activity Lactococcus lactis
5.3.1.5 S247A/S388T 27% of wild-type activity Lactococcus lactis
5.3.1.5 S388T insoluble and 8% of wild-type activity Lactococcus lactis
5.3.1.5 S388T/K407E soluble and 50% of wild-type activity Lactococcus lactis
5.3.1.5 T388S 11% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 Y218D 14% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 Y218D/V275A 24% of strain IO-1 wild-type activity Lactococcus lactis
5.3.1.5 Y275A 24% of strain IO-1 wild-type activity Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 2.25
-
 D-xylose wild-type Lactococcus lactis
5.3.1.5 3.46
-
 D-xylose mutant R202M/Y218D/V275A Lactococcus lactis
5.3.1.5 4.4
-
 D-xylose mutant S388T/K407E Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 49000
-
 SDS-PAGE Lactococcus lactis
5.3.1.5 49700
-
 calculated from amino acid sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Lactococcus lactis
-
 D-Xylulose
-
 ?
5.3.1.5 D-Xylose Lactococcus lactis IO-1 (Xyl+)
-
 D-Xylulose
-
 ?
5.3.1.5 D-Xylose Lactococcus lactis 210 (Xyl-)
-
 D-Xylulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Lactococcus lactis
-
-
-
5.3.1.5 Lactococcus lactis 210 (Xyl-)
-
-
-
5.3.1.5 Lactococcus lactis IO-1 (Xyl+)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5 purified enzyme is insoluble and inactive Lactococcus lactis
5.3.1.5 purified enzyme is soluble Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Xylose
-
 Lactococcus lactis D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Lactococcus lactis IO-1 (Xyl+) D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Lactococcus lactis 210 (Xyl-) D-Xylulose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 XI
-
 Lactococcus lactis
5.3.1.5 xylose isomerase
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 65
-
-
 Lactococcus lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.5 74
-
 activity decreases dramatically above 74°C Lactococcus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 124
-
 D-xylose mutant S388T/K407E Lactococcus lactis
5.3.1.5 142
-
 D-xylose mutant R202M/Y218D/V275A Lactococcus lactis
5.3.1.5 184
-
 D-xylose wild-type Lactococcus lactis