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Literature summary extracted from

  • Takahashi, H.; Inagaki, E.; Fujimoto, Y.; Kuroishi, C.; Nodake, Y.; Nakamura, Y.; Arisaka, F.; Yutani, K.; Kuramitsu, S.; Yokoyama, S.; Yamamoto, M.; Miyano, M.; Tahirov, T.H.
    Structure and implications for the thermal stability of phosphopantetheine adenylyltransferase from Thermus thermophilus (2004), Acta Crystallogr. Sect. D, 60, 97-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.3 expressed in Escherichia coli BL21 (DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.3 oil batch method Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.3 17700
-
 6 * 17700, crystal structure analysis, analytical ultracentrifugation data of the native enzyme suggests an equilibrium of two trimers and one hexamer in solution Thermus thermophilus
2.7.7.3 17800
-
 6 * 17800 Escherichia coli
2.7.7.3 67000
-
 analytical ultracentrifugation Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate Thermus thermophilus rate-limiting penultimate step in coenzyme A biosynthesis diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate Escherichia coli rate-limiting penultimate step in coenzyme A biosynthesis diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 rate-limiting penultimate step in coenzyme A biosynthesis diphosphate + 3'-dephospho-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.3 Escherichia coli
-
-
-
2.7.7.3 Thermus thermophilus
-
-
-
2.7.7.3 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.3
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Thermus thermophilus diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Escherichia coli diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate rate-limiting penultimate step in coenzyme A biosynthesis Thermus thermophilus diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate rate-limiting penultimate step in coenzyme A biosynthesis Escherichia coli diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate rate-limiting penultimate step in coenzyme A biosynthesis Thermus thermophilus HB8 / ATCC 27634 / DSM 579 diphosphate + 3'-dephospho-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.3 hexamer 6 * 17700, crystal structure analysis, analytical ultracentrifugation data of the native enzyme suggests an equilibrium of two trimers and one hexamer in solution Thermus thermophilus
2.7.7.3 hexamer 6 * 17800 Escherichia coli