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Literature summary extracted from

  • Kaushal, A.; Pabbi, S.; Sharma, P.
    Characterization of 2,3-butanediol-forming and valine-sensitive alpha-acetolactate synthase of Enterobacter cloacae (2003), World J. Microbiol. Biotechnol., 19, 487-493.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.2.1.6 glycerol, bovine serum albumin and pyruvate-thiamine diphosphate stabilize Enterobacter cloacae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 Ag+ 0.1 mM, 98% residual activity Enterobacter cloacae
2.2.1.6 Co2+ 0.1 mM, 44% residual activity Enterobacter cloacae
2.2.1.6 FAD 0.01 mM, 92% inhibition Enterobacter cloacae
2.2.1.6 Hg2+ 0.1 mM, 43% residual activity Enterobacter cloacae
2.2.1.6 Mg2+ 0.5 mM, 80% residual activity Enterobacter cloacae
2.2.1.6 Mn2+ 0.5 mM, 28% residual activity Enterobacter cloacae
2.2.1.6 NADP+ 0.01 mM, 63% inhibition Enterobacter cloacae
2.2.1.6 NADPH 0.01 mM, 100% inhibition Enterobacter cloacae
2.2.1.6 Sodium acetate
-
 Enterobacter cloacae
2.2.1.6 Sodium formate
-
 Enterobacter cloacae
2.2.1.6 sodium propionate
-
 Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 20
-
 pyruvate 37°C Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 60000
-
 3 * 60000, SDS-PAGE Enterobacter cloacae
2.2.1.6 200000
-
 gel filtration Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Enterobacter cloacae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 200
-
 37°C Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Enterobacter cloacae 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 trimer 3 * 60000, SDS-PAGE Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
-
 Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.6 30 40
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.6 6 8
-
 Enterobacter cloacae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.6 9
-
 80% residual activity Enterobacter cloacae