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Literature summary extracted from

  • Chappie, J.S.; Canaves, J.M.; Han, G.W.; Rife, C.L.; Xu, Q.; Stevens, R.C.
    The structure of a eukaryotic nicotinic acid phosphoribosyltransferase reveals structural heterogeneity among type II PRTases (2005), Structure, 13, 1385-1396.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.21 ATP functional modulation by ATP via autophosphorylation of His232, activation by ATP hydrolysis, determination of the binding site Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.21 expression of N-terminally tagged enzyme as selenomethionine-labeled variant in Escherichia coli strain DL41 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.21 recombinant selenomethionine-labeled enzyme, 10-30 mg/ml, in 10 mM Tris, pH 7.8, 150 mM NaCl, nanodroplet vapour diffusion method, 4°C, reservoir solution contains 6-16% PEG 5000 monomethyl ether, 0.06 M MES, and 0.04 M NaMES, pH 6.0, usage of 15-20% ethylene glycol as cryoprotectant, X-ray structure determination and analysis at 1.75 A resolution Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.21 additional information Saccharomyces cerevisiae molecular evolution mechanism ?
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Saccharomyces cerevisiae essential for the NAD salvage pathway beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Saccharomyces cerevisiae P39683 gene NPT1 at ocus YOR209C, eukaryotic type II enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.21 recombinant N-terminally tagged selenomethionine-labeled enzyme variant from Escherichia coli strain DL41 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate active site structure Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 additional information molecular evolution mechanism Saccharomyces cerevisiae ?
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O essential for the NAD salvage pathway Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
6.3.4.21 More enzyme is a member of the phosphoribosyltransferase superfamily or PRTase superfamily Saccharomyces cerevisiae
6.3.4.21 NAPRTase
-
 Saccharomyces cerevisiae
6.3.4.21 nicotinic acid phosphoribosyltransferase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.21 additional information
-
 additional information
-
 Saccharomyces cerevisiae