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Literature summary extracted from

  • DeMel, S.; Shi, J.; Martin, P.; Rosen, B.P.; Edwards, B.F.
    Arginine 60 in the ArsC arsenate reductase of E. coli plasmid R773 determines the chemical nature of the bound As(III) product (2004), Protein Sci., 13, 2330-2340.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.20.99.1 wild-type and mutant R60K and R60A enzymes in complex with product arsenite, X-ray diffraction structure determination and analysis at 1.3-1.8 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.20.99.1 R60A site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity Escherichia coli
1.20.99.1 R60K site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.99.1 arsenate + reduced acceptor Escherichia coli detoxification enzyme, arsenite is excreted or sequestered arsenite + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.99.1 Escherichia coli
-
 gene arsC, encoded on plasmid R773
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.20.99.1 arsenite + acceptor + H2O = arsenate + reduced acceptor the catalytic Cys12 of the wild-type enzyme, located at the active site and surrounded by the Arg triad with Arg60, Arg94, and Arg107, forms a unique monohydroxyl Cys12-thiol-arsenite adduct with a positive charge on the arsenic, which is an unstable intermediate Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.99.1 arsenate + reduced acceptor detoxification enzyme, arsenite is excreted or sequestered Escherichia coli arsenite + acceptor
-
 ?
1.20.99.1 arsenate + reduced acceptor the chemical nature of As(III) product is determined by Arg60 Escherichia coli arsenite + acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.20.99.1 ArsC
-
 Escherichia coli
1.20.99.1 arsenate reductase
-
 Escherichia coli