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Literature summary extracted from

  • Boulanger, M.J.; Murphy, M.E.
    Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: characterization of an active site isoleucine (2003), Protein Sci., 12, 248-256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.1
-
 Alcaligenes faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.1 crystals are grown at 19°C by hanging drop vapour diffusion using a reservoir of 100 mM sodium acetate, pH 4.7, 6%-10% polyethylene glycol 4000 and 1-5 mM cupric chloride, each drop is made from an equal volume of reservoir and a 15 mg/ml protein stock solution buffered in 10 mM Tris pH 7.0, crystals of mutants diffract to 1.8 A, nitrite-soaked oxidized crystals are obtained by placing crystals in reservoir solution supplemented with 5 mM sodium nitrite Alcaligenes faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.1 I257A 3.7% of wild-type activity Alcaligenes faecalis
1.7.2.1 I257G 2.5% of wild-type activity Alcaligenes faecalis
1.7.2.1 I257L 26% of wild-type activity Alcaligenes faecalis
1.7.2.1 I257M 4% of wild-type activity Alcaligenes faecalis
1.7.2.1 I257T 1.4% of wild-type activity Alcaligenes faecalis
1.7.2.1 I257V 125% of wild-type activity Alcaligenes faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.1 periplasm
-
 Alcaligenes faecalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper copper containing nitrite reductase Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 nitrite + ferrocytochrome c Alcaligenes faecalis
-
 NO + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c Alcaligenes faecalis S-6
-
 NO + H2O + ferricytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Alcaligenes faecalis
-
 S-6
-
1.7.2.1 Alcaligenes faecalis S-6
-
 S-6
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 417
-
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + ferrocytochrome c
-
 Alcaligenes faecalis NO + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c
-
 Alcaligenes faecalis S-6 NO + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + reduced methyl viologen
-
 Alcaligenes faecalis NO + oxidized methyl viologen + H2O
-
 ?
1.7.2.1 nitrite + reduced methyl viologen
-
 Alcaligenes faecalis S-6 NO + oxidized methyl viologen + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 AfNiR
-
 Alcaligenes faecalis