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Literature summary extracted from

  • Gu, X.; Chen, M.; Wang, Q.; Zhang, M.; Wang, B.; Wang, H.
    Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv (2005), Protein Expr. Purif., 42, 47-53.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.345 expression in Escherichia coli Mycobacterium tuberculosis
2.4.1.345 the pimA gene is cloned into the pET82a vector and the recombinant plasmid is transformed into Escherichia coli BL21 (DE3) Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.345 0.018
-
GDP-alpha-D-mannose pH 7.0, 37° Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.345 Mg2+ required, optimal concentration is 2-3 mM Mycobacterium tuberculosis
2.4.1.345 Mg2+ about 90% increase of activtiy in presence of 2-3 mM Mg2+ Mycobacterium tuberculosis
2.4.1.345 Mn2+ may partly substitute for Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.345 42000
-
-
Mycobacterium tuberculosis
2.4.1.345 42000
-
x * 42000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol Mycobacterium tuberculosis
-
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol Mycobacterium tuberculosis H37Rv
-
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol Mycobacterium tuberculosis PimA plays an essential role in the growth of mycobacteria GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol Mycobacterium tuberculosis H37Rv PimA plays an essential role in the growth of mycobacteria GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.345 Mycobacterium tuberculosis
-
H37Rv
-
2.4.1.345 Mycobacterium tuberculosis P9WMZ5
-
-
2.4.1.345 Mycobacterium tuberculosis H37Rv
-
H37Rv
-
2.4.1.345 Mycobacterium tuberculosis H37Rv P9WMZ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.345
-
Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.345 additional information
-
-
Mycobacterium tuberculosis
2.4.1.345 9.4
-
pH 7.0, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol
-
Mycobacterium tuberculosis GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol
-
Mycobacterium tuberculosis H37Rv GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol substrate bovine phosphatidylinositol Mycobacterium tuberculosis GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol substrate bovine phosphatidylinositol Mycobacterium tuberculosis H37Rv GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol
-
Mycobacterium tuberculosis GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol PimA plays an essential role in the growth of mycobacteria Mycobacterium tuberculosis GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol
-
Mycobacterium tuberculosis H37Rv GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?
2.4.1.345 GDP-mannose + phosphatidyl-myo-inositol PimA plays an essential role in the growth of mycobacteria Mycobacterium tuberculosis H37Rv GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.345 ? x * 42000, SDS-PAGE Mycobacterium tuberculosis
2.4.1.345 ? x * 42605, MALDI-TOF, x * 42739, calculated Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.345 GDP-mannosyltransferase
-
Mycobacterium tuberculosis
2.4.1.345 PimA
-
Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.345 7
-
-
Mycobacterium tuberculosis