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Literature summary extracted from

  • Zeng, J.; Li, D.
    Expression and purification of His-tagged rat mitochondrial 3-ketoacyl-CoA thiolase wild-type and His352 mutant proteins (2004), Protein Expr. Purif., 35, 320-326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.16 His-tagged wild-type and His352 mutant proteins overexpressed in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.16 H352A 3.3fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1154 times Rattus norvegicus
2.3.1.16 H352E 2.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1250 times Rattus norvegicus
2.3.1.16 H352K 3.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 526 times Rattus norvegicus
2.3.1.16 H352Y 3.1fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 429 times Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.16 0.021
-
 acetoacetyl-CoA pH 8.2, wild-type enzyme Rattus norvegicus
2.3.1.16 0.058
-
 acetoacetyl-CoA pH 8.2, mutant enzyme H352E Rattus norvegicus
2.3.1.16 0.065
-
 acetoacetyl-CoA pH 8.2, mutant enzyme H352Y Rattus norvegicus
2.3.1.16 0.07
-
 acetoacetyl-CoA pH 8.2, mutant enzyme H352A Rattus norvegicus
2.3.1.16 0.079
-
 acetoacetyl-CoA pH 8.2, mutant enzyme H352K Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.16 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 additional information Rattus norvegicus key enzyme involved in fatty acid oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.16 25
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.16 -80°C, wild-type and mutant enzymes H352E, H352A, H352K and H352Y, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 CoA + acetoacetyl-CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 additional information key enzyme involved in fatty acid oxidation Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.16 3-ketoacyl-CoA thiolase
-
 Rattus norvegicus