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Literature summary extracted from

  • Schofield, L.R.; Patchett, M.L.; Parker, E.J.
    Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus (2004), Protein Expr. Purif., 34, 17-27.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.54 resistant to denaturation by sodium dodecyl sulfate Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA inactivation, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus
2.5.1.54 additional information resistant to denaturation by sodium dodecyl sulfate, not inhibitory: phenylalanine, tyrosine, tryptophan Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.028
-
 D-erythrose 4-phosphate 60°C, pH 6.8 Pyrococcus furiosus
2.5.1.54 0.12
-
 phosphoenolpyruvate 60°C, pH 6.8 Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Mn2+ assay with 0.1 mM Pyrococcus furiosus
2.5.1.54 additional information EDTA-inactivated enzyme, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 5700
-
 gel filtration Pyrococcus furiosus
2.5.1.54 29224
-
 2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus
2.5.1.54 29226
-
 2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus
2.5.1.54 58450
-
 ESI-MS Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54
-
 Pyrococcus furiosus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.54 EDTA-inactivated enzyme, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 dimer 2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 DAH7PS
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.54 58
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 60
-
 30 min, presence of phosphoenolpyruvate, stable Pyrococcus furiosus
2.5.1.54 70
-
 30 min, presence of phosphoenolpyruvate, stable Pyrococcus furiosus
2.5.1.54 95
-
 presence of 0.1 mM Mn2+, 130 min, 50% residual activity, absence of Mn2+, 3 min, 50% residual activity Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 1.5
-
 phosphoenolpyruvate 60°C, pH 6.8 Pyrococcus furiosus