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Literature summary extracted from
- Methionine sulfoxide reductase regulation of yeast lifespan reveals reactive oxygen species-dependent and -independent components of aging (2004), Proc. Natl. Acad. Sci. USA, 101, 7999-8004.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | overexpression of MsrA in a yeast strain, expression of MsrA as N-terminally 6His-tagged protein in Escherichia coli strain BL-21 | Saccharomyces cerevisiae |
| 1.8.4.12 | overexpression of MsrB in a yeast strain, expression of MsrB as N-terminally 6His-tagged protein in Escherichia coli strain BL-21 | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrA specifically reduces the S-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae BY4741 | MsrA specifically reduces the S-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | Saccharomyces cerevisiae | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? | |
| 1.8.4.11 | additional information | Saccharomyces cerevisiae BY4741 | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrB specifically reduces the R-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Saccharomyces cerevisiae BY4741 | MsrB specifically reduces the R-form of methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | Saccharomyces cerevisiae | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? | |
| 1.8.4.12 | additional information | Saccharomyces cerevisiae BY4741 | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Saccharomyces cerevisiae | - |
enzyme MsrA | - |
| 1.8.4.11 | Saccharomyces cerevisiae BY4741 | - |
enzyme MsrA | - |
| 1.8.4.12 | Saccharomyces cerevisiae | - |
enzyme MsrB | - |
| 1.8.4.12 | Saccharomyces cerevisiae BY4741 | - |
enzyme MsrB | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | additional information | - |
recombinant activity in overexpressing yeast cells | Saccharomyces cerevisiae |
| 1.8.4.12 | additional information | - |
recombinant activity in overexpressing yeast cells | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | MsrA specifically reduces the S-form of methionine sulfoxide | Saccharomyces cerevisiae BY4741 | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae | ? | - |
? | |
| 1.8.4.11 | additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| 1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin | MsrB specifically reduces the R-form of methionine sulfoxide | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin | MsrB specifically reduces the R-form of methionine sulfoxide | Saccharomyces cerevisiae BY4741 | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | MsrB specifically reduces the R-form of methionine sulfoxide | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | MsrB specifically reduces the R-form of methionine sulfoxide | Saccharomyces cerevisiae BY4741 | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae | ? | - |
? | |
| 1.8.4.12 | additional information | the enzyme is essential in protection of the cells against oxidative damage by reactive oxygen species, yeast cell life span analysis of wild-type and mutant cells, the latter either overexpress or lack enzyme activity, overview | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
| 1.8.4.11 | MsrA | - |
Saccharomyces cerevisiae |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
| 1.8.4.12 | MsrB | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 37 | - |
assay at | Saccharomyces cerevisiae |
| 1.8.4.12 | 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
| 1.8.4.12 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Saccharomyces cerevisiae | |
| 1.8.4.12 | thioredoxin | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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