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Literature summary extracted from

  • Guo, M.; Bhaskar, B.; Li, H.; Barrows, T.P.; Poulos, T.L.
    Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link (2004), Proc. Natl. Acad. Sci. USA, 101, 5940-5945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.5 expression of V197C/C128A mutant in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.5 with comercial kit Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.5 V197C/C128A as active as the wild-type enzyme. Used to generate a covalent complex with a mutant cytochrome c Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.5 ferrocytochrome c + H2O2 Saccharomyces cerevisiae
-
ferricytochrome c + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.5 high-purity V197C/C128A mutant is obtained after an anion-exchange chromatography and gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.5 ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae ferricytochrome c + H2O
-
?
1.11.1.5 horse heart ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae horse heart ferricytochrome c + H2O
-
r
1.11.1.5 yeast ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae yeast ferricytochrome c + H2O
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.5 4.2
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
1.11.1.5 15.7
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
1.11.1.5 20.7
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
1.11.1.5 76.2
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
1.11.1.5 166.8
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
1.11.1.5 175.1
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
1.11.1.5 219.1
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
1.11.1.5 240.9
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
1.11.1.5 803.4
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
1.11.1.5 850.3
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
1.11.1.5 1167
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
1.11.1.5 1362
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae