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Literature summary extracted from

  • Fukatsu, H.; Hashimoto, Y.; Goda, M.; Higashibata, H.; Kobayashi, M.
    Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning (2004), Proc. Natl. Acad. Sci. USA, 101, 13726-13731.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.91 8-hydroxyquinoline 54% inhibition Arthrobacter pascens
3.5.1.91 AgNO3 0.25 mM, complete inhibition Arthrobacter pascens
3.5.1.91 CuCl 0.25 mM, complete inhibition Arthrobacter pascens
3.5.1.91 CuCl2 0.25 mM, complete inhibition Arthrobacter pascens
3.5.1.91 dithiothreitol 90% inhibition Arthrobacter pascens
3.5.1.91 HgCl2 0.25 mM, complete inhibition Arthrobacter pascens
3.5.1.91 N-ethylmaleimide complete inhibition Arthrobacter pascens
3.5.1.91 o-phenanthroline 38% inhibition Arthrobacter pascens
3.5.1.91 p-chloromercuribenzoate complete inhibition Arthrobacter pascens
3.5.1.91 phenylhydrazine 25% inhibition Arthrobacter pascens
3.5.1.91 SnCl2 0.25 mM, 34% inhibition Arthrobacter pascens
3.5.1.91 ZnCl2 0.25 mM, 25% inhibition Arthrobacter pascens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.91 0.075
-
 N-benzylformamide
-
 Arthrobacter pascens
3.5.1.91 7.5
-
 N-butylformamide
-
 Arthrobacter pascens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.91 Zn2+ 2.94 mol per mol of subunit Arthrobacter pascens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.91 61000
-
 2 * 61000, SDS-PAGE Arthrobacter pascens
3.5.1.91 121000
-
 gel filtration Arthrobacter pascens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.91 N-benzylformamide + H2O Arthrobacter pascens
-
 formate + benzylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.91 Arthrobacter pascens Q68AP4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.91 ammonium sulfate, DEAE-Sephacel, Resource ISO, Ressource Q Arthrobacter pascens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.91 28.9
-
-
 Arthrobacter pascens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.91 N-benzylformamide + H2O
-
 Arthrobacter pascens formate + benzylamine
-
 ?
3.5.1.91 N-butylformamide + H2O 3.4% of activity with N-benzylformamide Arthrobacter pascens formate + butylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.91 dimer 2 * 61000, SDS-PAGE Arthrobacter pascens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.91 NfdA
-
 Arthrobacter pascens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.91 35
-
-
 Arthrobacter pascens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.91 30
-
 2% loss of activity after 30 min Arthrobacter pascens
3.5.1.91 35
-
 6% loss of activity after 30 min Arthrobacter pascens
3.5.1.91 40
-
 16% loss of activity after 30 min Arthrobacter pascens
3.5.1.91 45
-
 25% loss of activity after 30 min Arthrobacter pascens
3.5.1.91 50
-
 49% loss of activity after 30 min Arthrobacter pascens
3.5.1.91 55
-
 complete loss of activity after 30 min Arthrobacter pascens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.91 7
-
-
 Arthrobacter pascens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.91 7.5 8.5 stable Arthrobacter pascens