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Literature summary extracted from

  • Sian, H.K.; Said, M.; Hassan, O.; Kamaruddin, K.; Ismail, A.F.; Rahman, R.A.; Mahmood, N.A.N.; Illias, R.M.
    Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 (2004), Proc. Biochem., 40, 1101-1111.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.19 Ca2+, 20 mM, increases thermal stability Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 CoCl2 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 CuSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 EDTA 1 mM, 83% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 FeCl3 1 mM, 70% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 FeSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 K2SO4 1 mM, 54% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 MgCl2 1 mM, 53% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 MgSO4 1 mM, 65% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 NaCl 1 mM, 28% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 NaNO3 1 mM, 54% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 SDS 1 mM, 55% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 ZnCl2 1 mM, 73% inhibition Bacillus sp. (in: Bacteria)
2.4.1.19 ZnSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 FeCl2 1 mM, 89.3% activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 75000
-
 1 * 75000, SDS-PAGE Bacillus sp. (in: Bacteria)
2.4.1.19 79000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 G1
-
2.4.1.19 Bacillus sp. (in: Bacteria) G1
-
 G1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor tapioca starch is the best substrate Bacillus sp. (in: Bacteria) beta-cyclodextrin + gamma-cyclodextrin production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents ?
2.4.1.19 soluble starch + glycosyl acceptor tapioca starch is the best substrate Bacillus sp. (in: Bacteria) G1 beta-cyclodextrin + gamma-cyclodextrin production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 monomer 1 * 75000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 50 70 50°C: about 15% of maximal activity, 70°C: about 20% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 60
-
 pH 6.0, 30 min, stable up to Bacillus sp. (in: Bacteria)
2.4.1.19 70
-
 in presence of 20 mM Ca2+, stable up to Bacillus sp. (in: Bacteria)
2.4.1.19 76
-
 pH 6.0, 30 min, 50% loss of activity Bacillus sp. (in: Bacteria)
2.4.1.19 80
-
 pH 6.0, 30 min, 86% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 6
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 5 7 pH 5.0: about 25% of maximal activity, pH 7.0: about 60% of maximal activity, the enzyme does not show any activity at pH 4.0 and at pH 10.0 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 7 9 60°C, 30 min, stable Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.19 Bacillus sp. (in: Bacteria)
-
-
 8.8