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Literature summary extracted from

  • Takeda, T.; Fry, S.C.
    Control of xyloglucan endotransglucosylase activity by salts and anionic polymers (2004), Planta, 219, 722-732.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.207 acidic arabinogalactan protein MW about 5000 Da, acidic polymers and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth, composition overview Brassica oleracea
2.4.1.207 Gum arabic from Acacia sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea
2.4.1.207 hypochlorite-oxidized xyloglucan from tamarind, 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea
2.4.1.207 additional information no activation by carboxymethylcellulose, non-oxidized xyloglucan, homo-galacturonic acid, and starch, overview Brassica oleracea
2.4.1.207 Pectin from Citrus sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea

General Stability

EC Number General Stability Organism
2.4.1.207 activity is lost after partial purification and desalting, ascorbic acid and 2-mercaptoethanol cannot prevent the loss, activity can be restored by addition of a wide variety of inorganic and organic salts Brassica oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 4-O-methyl-glucuronoxylan
-
 Brassica oleracea
2.4.1.207 AlCl3 inhibitory at concentrations above 0.1 mM Brassica oleracea
2.4.1.207 alginate alpha-carrageenan from red algae Gigartina ssp. Brassica oleracea
2.4.1.207 CaCl2 inhibitory at concentrations above 0.1 mM Brassica oleracea
2.4.1.207 homogalacturonan
-
 Brassica oleracea
2.4.1.207 LaCl3 inhibitory at 5 mM Brassica oleracea
2.4.1.207 MgCl2 inhibitory at concentrations above 0.1 mM Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall
-
 Brassica oleracea 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.207 Al3+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
2.4.1.207 Ca2+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
2.4.1.207 K+ best at about 300 mM Brassica oleracea
2.4.1.207 La3+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
2.4.1.207 Mg2+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
2.4.1.207 additional information the enzyme is activated by a wide variety of inorganic and organic salts Brassica oleracea
2.4.1.207 Na+ best at about 300 mM Brassica oleracea
2.4.1.207 NH4+ best at about 300 mM Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 additional information Brassica oleracea acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth ?
-
 ?
2.4.1.207 xyloglucan oligosaccharides + xyloglucan Brassica oleracea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Brassica oleracea
-
 cauliflower
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 partial Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 floret
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.207 additional information
-
-
 Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 additional information acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth Brassica oleracea ?
-
 ?
2.4.1.207 xyloglucan oligosaccharides + xyloglucan
-
 Brassica oleracea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET
-
 Brassica oleracea
2.4.1.207 xyloglucan endotransglucosylase
-
 Brassica oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 27
-
 assay at Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5.5
-
 assay at Brassica oleracea