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Literature summary extracted from

  • Vigara, J.; Garcia-Sanchez, M.I.; Garbayo, I.; Vilchez, C.; Vega, J.M.
    Purification and characterization of ferredoxin-nitrite reductase from the eukaryotic microalga Monoraphidium braunii (2002), Plant Physiol. Biochem., 40, 401-405.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.1
-
 oxidized ferredoxin 40°C, pH 7.5 Chlorolobion braunii
1.7.7.1 0.26
-
 methyl viologen 40°C, pH 7.5 Chlorolobion braunii
1.7.7.1 0.7
-
 nitrite 40°C, pH 7.5 Chlorolobion braunii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.1 Fe contains siroheme and Fe-S clusters Chlorolobion braunii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 63000
-
 1 * 63000, SDS-PAGE Chlorolobion braunii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin Chlorolobion braunii
-
 NH3 + H2O + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Chlorolobion braunii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1 protamine sulfate, DEAE-Sephacel, Phenyl-Sepharose, DEAE-Sephacel Chlorolobion braunii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 214.4
-
-
 Chlorolobion braunii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 NH3 + H2O + oxidized ferredoxin
-
 Chlorolobion braunii nitrite + reduced ferredoxin + H+
-
 r
1.7.7.1 nitrite + reduced ferredoxin
-
 Chlorolobion braunii NH3 + H2O + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced methyl viologen
-
 Chlorolobion braunii NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 monomer 1 * 63000, SDS-PAGE Chlorolobion braunii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.1 40
-
-
 Chlorolobion braunii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.1 7.5
-
-
 Chlorolobion braunii

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.1 siroheme
-
 Chlorolobion braunii