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Literature summary extracted from

  • Duan, G.L.; Zhu, Y.G.; Tong, Y.P.; Cai, C.; Kneer, R.
    Characterization of arsenate reductase in the extract of roots and fronds of Chinese brake fern, an arsenic hyperaccumulator (2005), Plant Physiol., 138, 461-469.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.20.4.1 additional information enzyme is arsenate-inducible Pteris vittata

Protein Variants

EC Number Protein Variants Comment Organism
1.20.4.1 additional information a disruption mutant is inactive Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.4.1 arsenite noncompetitive Pteris vittata
1.20.4.1 phosphate competitive Pteris vittata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.1 0.57
-
 reduced glutathione pH 6.5, 30°C Pteris vittata
1.20.4.1 2.3
-
 arsenate pH 6.5, 30°C Pteris vittata
1.20.4.1 2.33
-
 arsenate pH 6.5, 30°C Pteris vittata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.4.1 arsenate + reduced acceptor Arabidopsis thaliana
-
 arsenite + acceptor
-
 ?
1.20.4.1 arsenate + reduced acceptor Oryza sativa
-
 arsenite + acceptor
-
 ?
1.20.4.1 arsenate + reduced glutathione Pteris vittata enzyme plays an important role in detoxification of arsenate arsenite + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.1 Arabidopsis thaliana
-
-
-
1.20.4.1 Oryza sativa
-
-
-
1.20.4.1 Pteris vittata
-
 Chinese brake fern, an arsenic hyperaccumulator
-
1.20.4.1 Pteris vittata
-
 5fold increase in enzyme activity after 9 days treatment with 2 mM arsenate
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.20.4.1 arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H2O reaction mechanism Pteris vittata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.20.4.1 frond
-
 Pteris vittata
-
1.20.4.1 additional information no activity in shoot, arsenite is the main storage form in the fern fronds Pteris vittata
-
1.20.4.1 additional information no enzymic activity in shoots Pteris vittata
-
1.20.4.1 root
-
 Arabidopsis thaliana
-
1.20.4.1 root
-
 Oryza sativa
-
1.20.4.1 root
-
 Pteris vittata
-
1.20.4.1 shoot
-
 Arabidopsis thaliana
-
1.20.4.1 shoot
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.20.4.1 additional information
-
 in vivo activity after feeding experiments Pteris vittata
1.20.4.1 2
-
 shoot Arabidopsis thaliana
1.20.4.1 2.45
-
 root Oryza sativa
1.20.4.1 3.55
-
 root Arabidopsis thaliana
1.20.4.1 3.84
-
 shoot Oryza sativa
1.20.4.1 4.8
-
 isolated roots in arsenate containiing culture medium, basic level Pteris vittata
1.20.4.1 24
-
 isolated roots in arsenate containiing culture medium, induced for 9 h by arsenate Pteris vittata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.1 arsenate + reduced acceptor
-
 Arabidopsis thaliana arsenite + acceptor
-
 ?
1.20.4.1 arsenate + reduced acceptor
-
 Oryza sativa arsenite + acceptor
-
 ?
1.20.4.1 arsenate + reduced glutathione
-
 Arabidopsis thaliana arsenite + glutathione
-
 ?
1.20.4.1 arsenate + reduced glutathione
-
 Oryza sativa arsenite + glutathione
-
 ?
1.20.4.1 arsenate + reduced glutathione
-
 Pteris vittata arsenite + glutathione
-
 ?
1.20.4.1 arsenate + reduced glutathione enzyme plays an important role in detoxification of arsenate Pteris vittata arsenite + glutathione
-
 ?
1.20.4.1 additional information no substrate: phosphate, nitrate Pteris vittata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.20.4.1 AR
-
 Arabidopsis thaliana
1.20.4.1 AR
-
 Oryza sativa
1.20.4.1 AR
-
 Pteris vittata
1.20.4.1 arsenate reductase
-
 Arabidopsis thaliana
1.20.4.1 arsenate reductase
-
 Oryza sativa
1.20.4.1 arsenate reductase
-
 Pteris vittata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.4.1 20 50 broad optimum Pteris vittata
1.20.4.1 30
-
 assay at Arabidopsis thaliana
1.20.4.1 30
-
 assay at Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.20.4.1 20 50
-
 Pteris vittata
1.20.4.1 20 50 broad Pteris vittata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.4.1 6.5
-
-
 Pteris vittata
1.20.4.1 6.5
-
 broad optimum Pteris vittata
1.20.4.1 6.5
-
 assay at Arabidopsis thaliana
1.20.4.1 6.5
-
 assay at Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.20.4.1 5.5 7
-
 Pteris vittata
1.20.4.1 5.5 7.5 90% of maximal activity at pH 5.5, 60% at pH 7.5 Pteris vittata
1.20.4.1 5.5
-
 90% of maximum activity Pteris vittata
1.20.4.1 7.5
-
 60% of maximum activity Pteris vittata

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.1 glutathione
-
 Arabidopsis thaliana
1.20.4.1 glutathione
-
 Oryza sativa
1.20.4.1 glutathione
-
 Pteris vittata