BRENDA - Enzyme Database show

Characterization of arsenate reductase in the extract of roots and fronds of Chinese brake fern, an arsenic hyperaccumulator

Duan, G.L.; Zhu, Y.G.; Tong, Y.P.; Cai, C.; Kneer, R.; Plant Physiol. 138, 461-469 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
additional information
enzyme is arsenate-inducible
Pteris vittata
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.20.4.1
additional information
a disruption mutant is inactive
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
noncompetitive
Pteris vittata
1.20.4.1
phosphate
competitive
Pteris vittata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.57
-
reduced glutathione
pH 6.5, 30°C
Pteris vittata
1.20.4.1
2.3
-
arsenate
pH 6.5, 30°C
Pteris vittata
1.20.4.1
2.33
-
arsenate
pH 6.5, 30°C
Pteris vittata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced acceptor
Arabidopsis thaliana
-
arsenite + acceptor
-
-
?
1.20.4.1
arsenate + reduced acceptor
Oryza sativa
-
arsenite + acceptor
-
-
?
1.20.4.1
arsenate + reduced glutathione
Pteris vittata
enzyme plays an important role in detoxification of arsenate
arsenite + glutathione
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.4.1
Arabidopsis thaliana
-
-
-
1.20.4.1
Oryza sativa
-
-
-
1.20.4.1
Pteris vittata
-
5fold increase in enzyme activity after 9 days treatment with 2 mM arsenate; Chinese brake fern, an arsenic hyperaccumulator
-
Reaction
EC Number
Reaction
Commentary
Organism
1.20.4.1
arsenate + glutaredoxin = arsenite + glutaredoxin disulfide + H2O
reaction mechanism
Pteris vittata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.20.4.1
frond
-
Pteris vittata
-
1.20.4.1
additional information
no activity in shoot, arsenite is the main storage form in the fern fronds; no enzymic activity in shoots
Pteris vittata
-
1.20.4.1
root
-
Arabidopsis thaliana
-
1.20.4.1
root
-
Oryza sativa
-
1.20.4.1
root
-
Pteris vittata
-
1.20.4.1
shoot
-
Oryza sativa
-
1.20.4.1
shoot
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.4.1
additional information
-
in vivo activity after feeding experiments
Pteris vittata
1.20.4.1
2
-
shoot
Arabidopsis thaliana
1.20.4.1
2.45
-
root
Oryza sativa
1.20.4.1
3.55
-
root
Arabidopsis thaliana
1.20.4.1
3.84
-
shoot
Oryza sativa
1.20.4.1
4.8
-
isolated roots in arsenate containiing culture medium, basic level
Pteris vittata
1.20.4.1
24
-
isolated roots in arsenate containiing culture medium, induced for 9 h by arsenate
Pteris vittata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced acceptor
-
660258
Arabidopsis thaliana
arsenite + acceptor
-
-
-
?
1.20.4.1
arsenate + reduced acceptor
-
660258
Oryza sativa
arsenite + acceptor
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Arabidopsis thaliana
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Oryza sativa
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Pteris vittata
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
enzyme plays an important role in detoxification of arsenate
660258
Pteris vittata
arsenite + glutathione
-
-
-
?
1.20.4.1
additional information
no substrate: phosphate, nitrate
660258
Pteris vittata
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.4.1
20
50
broad optimum
Pteris vittata
1.20.4.1
30
-
assay at
Arabidopsis thaliana
1.20.4.1
30
-
assay at
Oryza sativa
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.20.4.1
20
50
; broad
Pteris vittata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
6.5
-
assay at
Arabidopsis thaliana
1.20.4.1
6.5
-
assay at
Oryza sativa
1.20.4.1
6.5
-
; broad optimum
Pteris vittata
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.20.4.1
5.5
7
-
Pteris vittata
1.20.4.1
5.5
7.5
90% of maximal activity at pH 5.5, 60% at pH 7.5
Pteris vittata
1.20.4.1
5.5
-
90% of maximum activity
Pteris vittata
1.20.4.1
7.5
-
60% of maximum activity
Pteris vittata
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.20.4.1
glutathione
-
Arabidopsis thaliana
1.20.4.1
glutathione
-
Oryza sativa
1.20.4.1
glutathione
-
Pteris vittata
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
additional information
enzyme is arsenate-inducible
Pteris vittata
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.20.4.1
glutathione
-
Arabidopsis thaliana
1.20.4.1
glutathione
-
Oryza sativa
1.20.4.1
glutathione
-
Pteris vittata
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.20.4.1
additional information
a disruption mutant is inactive
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
noncompetitive
Pteris vittata
1.20.4.1
phosphate
competitive
Pteris vittata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.57
-
reduced glutathione
pH 6.5, 30°C
Pteris vittata
1.20.4.1
2.3
-
arsenate
pH 6.5, 30°C
Pteris vittata
1.20.4.1
2.33
-
arsenate
pH 6.5, 30°C
Pteris vittata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced acceptor
Arabidopsis thaliana
-
arsenite + acceptor
-
-
?
1.20.4.1
arsenate + reduced acceptor
Oryza sativa
-
arsenite + acceptor
-
-
?
1.20.4.1
arsenate + reduced glutathione
Pteris vittata
enzyme plays an important role in detoxification of arsenate
arsenite + glutathione
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.20.4.1
frond
-
Pteris vittata
-
1.20.4.1
additional information
no activity in shoot, arsenite is the main storage form in the fern fronds; no enzymic activity in shoots
Pteris vittata
-
1.20.4.1
root
-
Arabidopsis thaliana
-
1.20.4.1
root
-
Oryza sativa
-
1.20.4.1
root
-
Pteris vittata
-
1.20.4.1
shoot
-
Oryza sativa
-
1.20.4.1
shoot
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.4.1
additional information
-
in vivo activity after feeding experiments
Pteris vittata
1.20.4.1
2
-
shoot
Arabidopsis thaliana
1.20.4.1
2.45
-
root
Oryza sativa
1.20.4.1
3.55
-
root
Arabidopsis thaliana
1.20.4.1
3.84
-
shoot
Oryza sativa
1.20.4.1
4.8
-
isolated roots in arsenate containiing culture medium, basic level
Pteris vittata
1.20.4.1
24
-
isolated roots in arsenate containiing culture medium, induced for 9 h by arsenate
Pteris vittata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced acceptor
-
660258
Arabidopsis thaliana
arsenite + acceptor
-
-
-
?
1.20.4.1
arsenate + reduced acceptor
-
660258
Oryza sativa
arsenite + acceptor
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Arabidopsis thaliana
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Oryza sativa
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
-
660258
Pteris vittata
arsenite + glutathione
-
-
-
?
1.20.4.1
arsenate + reduced glutathione
enzyme plays an important role in detoxification of arsenate
660258
Pteris vittata
arsenite + glutathione
-
-
-
?
1.20.4.1
additional information
no substrate: phosphate, nitrate
660258
Pteris vittata
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.4.1
20
50
broad optimum
Pteris vittata
1.20.4.1
30
-
assay at
Arabidopsis thaliana
1.20.4.1
30
-
assay at
Oryza sativa
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.20.4.1
20
50
; broad
Pteris vittata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
6.5
-
assay at
Arabidopsis thaliana
1.20.4.1
6.5
-
assay at
Oryza sativa
1.20.4.1
6.5
-
; broad optimum
Pteris vittata
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.20.4.1
5.5
7
-
Pteris vittata
1.20.4.1
5.5
7.5
90% of maximal activity at pH 5.5, 60% at pH 7.5
Pteris vittata
1.20.4.1
5.5
-
90% of maximum activity
Pteris vittata
1.20.4.1
7.5
-
60% of maximum activity
Pteris vittata