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Literature summary extracted from

  • Schupp, N.; Ziegler, P.
    The relation of starch phosphorylases to starch metabolism in wheat (2004), Plant Cell Physiol., 45, 1471-1484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1 DNA sequence determination and analysis Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.03
-
 amylopectin pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.03
-
 amylopectin pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.04
-
 glycogen pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.07
-
 glycogen pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.1
-
 soluble starch pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.15
-
 soluble starch pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.44
-
 maltotetraose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.71
-
 amylose pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 0.81
-
 maltoheptaose pH 7.5, 30°C, degradation reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 3.01
-
 amylose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum
2.4.1.1 4.49
-
 maltoheptaose pH 7.5, 37°C, synthesis reaction, cytosolic isozyme Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast plastidic isozyme Triticum aestivum 9507
-
2.4.1.1 cytosol cytosolic isozyme Triticum aestivum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 91500
-
 x * 91500, cytosolic isozyme, SDS-PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate Triticum aestivum the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Triticum aestivum Q9LKJ3 cytosolic isozyme; var. Star, 3 tissue-dependent enzyme isoforms P1, P2, and P3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 cytosolic isozyme from leaves 7140fold to homogeneity by affinity chromatography Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 embryo contains only cytosolic isozyme Triticum aestivum
-
2.4.1.1 endosperm
-
 Triticum aestivum
-
2.4.1.1 leaf young and mature, the latter contain only the plastidic isozyme Triticum aestivum
-
2.4.1.1 seed germinated seeds contain only the cytosolic isozyme restricted to the embryo Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 additional information
-
 substrate specificity of cytosolic isozyme Triticum aestivum
2.4.1.1 57.2
-
 purified cytosolic isozyme Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate the cytosolic isozyme is involved in the processing of incoming carbohydrate during rapid tissue growth, plastidic isozyme is associated with transitory leaf starch metabolism and with the initiation of seed endosperm reserve starch accumulation, but it plays no role in the degradation of the reserve starch Triticum aestivum (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate the cytosolic isozyme utilizes amylopectin, amylose, soluble starch, and glycogen as polysaccharide substrates, while the plastidic isozyme prefers starch Triticum aestivum (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 amylopectin + phosphate
-
 Triticum aestivum amylopectin + D-glucose-1-phosphate
-
 r
2.4.1.1 glycogen + phosphate
-
 Triticum aestivum alpha-D-glucan + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltoheptaose + alpha-D-glucose 1-phosphate the cytosolic isozyme Triticum aestivum maltooctaose + phosphate
-
 r
2.4.1.1 maltoheptaose + phosphate the cytosolic isozyme Triticum aestivum maltohexaose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltotetraose + alpha-D-glucose 1-phosphate the cytosolic isozyme Triticum aestivum maltopentaose + phosphate
-
 r
2.4.1.1 additional information substrate specificity of cytosolic isozyme Triticum aestivum ?
-
 ?
2.4.1.1 soluble starch + phosphate
-
 Triticum aestivum ? + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 91500, cytosolic isozyme, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 alpha-1,4-glycan phosphorylase
-
 Triticum aestivum
2.4.1.1 glycogen phosphorylase
-
 Triticum aestivum
2.4.1.1 starch phosphorylase
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 30
-
 degradation reaction, assay at Triticum aestivum
2.4.1.1 37
-
 synthesis reaction, assay at Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 7.5
-
 assay at Triticum aestivum