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Literature summary extracted from

  • Boldt, R.; Edner, C.; Kolukisaoglu, U.; Hagemann, M.; Weckwerth, W.; Wienkoop, S.; Morgenthal, K.; Bauwe, H.
    D-Glycerate 3-kinase, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family (2005), Plant Cell, 17, 2413-2420.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.31 functional expression of His-tagged enzyme in Escherichia coli strain BL21, phylogenetic analysis Saccharomyces cerevisiae
2.7.1.31 single-copy gene, partial DNA and amino acid sequence determination and analysis, functional expression of His-tagged enzyme in Escherichia coli strain BL21, phylogenetic analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.31 additional information construction of inactive T-DNA insertion knockout mutants, which are not viable in normal air, but grow under elevated CO2 concentration, and show high accumulation of glycerate, overview Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.31 ammonium sulfate inhibits at high concentrations Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.31 39000
-
 x * 39000, native enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.31 ATP + D-glycerate Saccharomyces cerevisiae concluding reaction of the photorespiratory C2 cycle essential for living in oxygenic atmosphere, overview ADP + 3-phospho-D-glycerate
-
 ?
2.7.1.31 ATP + D-glycerate Arabidopsis thaliana concluding reaction of the photorespiratory C2 cycle essential for plants to live in oxygenic atmosphere, overview ADP + 3-phospho-D-glycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.31 Arabidopsis thaliana
-
 single-copy gene
-
2.7.1.31 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.31 native enzyme 867fold from leaves by hydrophobic interaction, anion exchange, and 2 steps affinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Arabidopsis thaliana
2.7.1.31 recombinant His-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.31 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.31 21.3
-
 purified recombinant enzyme Saccharomyces cerevisiae
2.7.1.31 27.9
-
 purified recombinant enzyme Arabidopsis thaliana
2.7.1.31 54.6
-
 purified native enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.31 ATP + D-glycerate
-
 Saccharomyces cerevisiae ADP + 3-phospho-D-glycerate
-
 ?
2.7.1.31 ATP + D-glycerate concluding reaction of the photorespiratory C2 cycle essential for living in oxygenic atmosphere, overview Saccharomyces cerevisiae ADP + 3-phospho-D-glycerate
-
 ?
2.7.1.31 ATP + D-glycerate concluding reaction of the photorespiratory C2 cycle essential for plants to live in oxygenic atmosphere, overview Arabidopsis thaliana ADP + 3-phospho-D-glycerate
-
 ?
2.7.1.31 ATP + D-glycerate the enzyme contains an ATP/GTP binding site motif A, a P-loop, domain Arabidopsis thaliana ADP + 3-phospho-D-glycerate
-
 ?
2.7.1.31 additional information no activity with uridine and ribulose 5-phosphate Saccharomyces cerevisiae ?
-
 ?
2.7.1.31 additional information no activity with uridine and ribulose 5-phosphate Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.31 ? x * 39000, native enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.31 D-glycerate kinase
-
 Saccharomyces cerevisiae
2.7.1.31 D-glycerate kinase
-
 Arabidopsis thaliana
2.7.1.31 GLYK
-
 Saccharomyces cerevisiae
2.7.1.31 GLYK
-
 Arabidopsis thaliana
2.7.1.31 More the enzyme belongs to the glycerate kinase family of enzymes Saccharomyces cerevisiae
2.7.1.31 More the enzyme belongs to the glycerate kinase family of enzymes Arabidopsis thaliana