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Literature summary extracted from

  • Urahara, T.; Tsuchiya, K.; Kotake, T.; Tohno-oka, T.; Komae, K.; Kawada, N.; Tsumuraya, Y.
    A beta-(1->4)-xylosyltransferase involved in the synthesis of arabinoxylans in developing barley endosperms (2004), Physiol. Plant., 122, 169-180.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.24 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate i.e. CHAPS, slight activation Hordeum vulgare
2.4.2.24 Brij-35 slight activation Hordeum vulgare
2.4.2.24 additional information enzyme requires detergents for activity Hordeum vulgare
2.4.2.24 Triton X-100 high activation at 0.5% Hordeum vulgare
2.4.2.24 Triton X-102
-
 Hordeum vulgare
2.4.2.24 Tween 20
-
 Hordeum vulgare
2.4.2.24 Zwittergent 3-16
-
 Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.24 Al3+
-
 Hordeum vulgare
2.4.2.24 Ca2+
-
 Hordeum vulgare
2.4.2.24 Cu2+
-
 Hordeum vulgare
2.4.2.24 Fe3+
-
 Hordeum vulgare
2.4.2.24 Hg2+
-
 Hordeum vulgare
2.4.2.24 n-octyl-beta-D-glucoside 50% inhibition Hordeum vulgare
2.4.2.24 sodium cholate complete inhibition Hordeum vulgare
2.4.2.24 sodium deoxycholate complete inhibition Hordeum vulgare
2.4.2.24 Zn2+
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.24 2.1
-
 pyridylaminated beta-(1,4)-xylotriose pH 6.8, 20°C Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.24 membrane
-
 Hordeum vulgare 16020
-
2.4.2.24 microsome
-
 Hordeum vulgare
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.24 Mg2+ enzyme requires Mg2+ or Mn2+ for activity, activates Hordeum vulgare
2.4.2.24 Mn2+ enzyme requires Mg2+ or Mn2+ for activity, high activation at 5 mM MnCl2 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.24 additional information Hordeum vulgare metabolic flux control and alterations during light reduction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.24 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.24 preparation of microsomal fraction Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.24 endosperm enzyme activity varies according to developmental stage of the endosperm, overview Hordeum vulgare
-
2.4.2.24 additional information no activity in mature seeds, no correlation between enzyme activity and xylan content in cell walls of different cultivars Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.24 0.000075 0.0004 xylan polymers Hordeum vulgare
2.4.2.24 0.002
-
 xyloseoligosaccharides Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.24 additional information substrate specificity Hordeum vulgare ?
-
 ?
2.4.2.24 additional information metabolic flux control and alterations during light reduction Hordeum vulgare ?
-
 ?
2.4.2.24 pyridylaminated beta-(1,4)-xylotriose + UDP-D-xylose
-
 Hordeum vulgare pyridylaminated beta-(1,4)-xylotetraose + UDP
-
 ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)7
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)8
-
 ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)8
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)9
-
 ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)9
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)10
-
 ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)n substrate are e.g. higher xylooligosaccharide or xylans from different sources, chain elongation by formation of beta(1,4)-linkages, xylooligosaccharide substrates can be pyrimidylaminated or derivatized with 2-aminopyridine at their reducing ends and still serve as substrates, substrate can contain single 4-O-methyl-glucuronic acid residues Hordeum vulgare UDP + (1,4-beta-D-xylan)n+1
-
 ?
2.4.2.24 UDP-D-xylose + 4-O-methyl-glucuronoxylan
-
 Hordeum vulgare UDP + ?
-
 ?
2.4.2.24 UDP-D-xylose + arabinoxylan
-
 Hordeum vulgare UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.24 beta-(1,4)-xylosyltransferase
-
 Hordeum vulgare
2.4.2.24 XylTase
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.24 20
-
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.24 6.8
-
-
 Hordeum vulgare