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Literature summary extracted from
- Methionine sulphoxide reductase is an important antioxidant enzyme in the gastric pathogen Helicobacter pylori (2004), Mol. Microbiol., 53, 1397-1406.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | msr gene, DNA sequence determination and analysis, subcloning in Escherichia coli strain DH5-alpha, functional complementation of the enzyme-deficient mutant with the wild-type gene | Helicobacter pylori |
| 1.8.4.12 | msr gene, DNA sequence determination and analysis, subcloning in Escherichia coli strain DH5-alpha, functional complementation of the enzyme-deficient mutant with the wild-type gene | Helicobacter pylori |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | additional information | construction of an enzyme-deficient mutant strain which shows diminished growth in presence of chemical oxidants with rapid loss of viability compared to the wild-type strain, activity can be recovered by complementation with the wild-type gene, study of oxidative stress resistance and colonization activity | Helicobacter pylori |
| 1.8.4.12 | additional information | construction of an enzyme-deficient mutant strain which shows diminished growth in presence of chemical oxidants with rapid loss of viability compared to the wild-type strain, activity can be recovered by complementation with the wild-type gene, study of oxidative stress resistance and colonization activity | Helicobacter pylori |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.4.11 | membrane | - |
Helicobacter pylori | 16020 | - |
| 1.8.4.11 | additional information | no activity in the cytoplasm | Helicobacter pylori | - |
- |
| 1.8.4.12 | membrane | - |
Helicobacter pylori | 16020 | - |
| 1.8.4.12 | additional information | no activity in the cytoplasm | Helicobacter pylori | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 43000 | - |
x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
| 1.8.4.12 | 43000 | - |
x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Helicobacter pylori | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Helicobacter pylori | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Helicobacter pylori | - |
enzyme forms MsrA and MsrB are fused together forming a single enzyme termed Msr | - |
| 1.8.4.12 | Helicobacter pylori | - |
enzyme forms MsrA and MsrB are fused together forming a single protein termed Msr | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | - |
Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | the enzyme also exhibits MsrB activity utilizing L-methionine (R)-sulfoxide as substrate | Helicobacter pylori | ? | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | - |
Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | the enzyme also exhibits MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | Helicobacter pylori | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | ? | x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
| 1.8.4.12 | ? | x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulphoxide reductase | - |
Helicobacter pylori |
| 1.8.4.11 | MSR | - |
Helicobacter pylori |
| 1.8.4.12 | methionine sulphoxide reductase | - |
Helicobacter pylori |
| 1.8.4.12 | MSR | - |
Helicobacter pylori |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.4 | - |
assay at | Helicobacter pylori |
| 1.8.4.12 | 7.4 | - |
assay at | Helicobacter pylori |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Helicobacter pylori | |
| 1.8.4.12 | thioredoxin | - |
Helicobacter pylori | |
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