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Literature summary extracted from

  • Unsoeld, I.A.; Li, S.M.
    Overproduction, purification and characterization of FgaPT2, a dimethylallyltryptophan synthase from Aspergillus fumigatus (2005), Microbiology, 151, 1499-1505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 gene fgaPT2, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Saccharomyces cerevisiae INVSc1 Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.004
-
 dimethylallyl diphosphate
-
 Aspergillus fumigatus
2.5.1.34 0.008
-
 L-tryptophan
-
 Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.34 soluble
-
 Aspergillus fumigatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 Ca2+ activates, but is not essential for activity Aspergillus fumigatus
2.5.1.34 Mg2+ activates, but is not essential for activity Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 52500
-
 2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
2.5.1.34 57000
-
 2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
2.5.1.34 86000 91000 gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus first key step in ergot alkaloid biosynthesis providing the pharmaceutically relevant compounds lysergic acid, ergotamine, fumigaclavine A-C, festuclavine, and elymoclavine diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus Q50EL0 strain AfA 32C2 and strain B5233, ATCC 13073, gene fgaPT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34 recombinant His6-tagged enzyme to near homogeneity from Saccharomyces cerevisiae by nickel affinity chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 5-methyl-DL-tryptophan 11.6% of the activity with L-tryptophan Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
-
 ir
2.5.1.34 dimethylallyl diphosphate + 6-methyl-DL-tryptophan 6.8% of the activity with L-tryptophan Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
-
 ir
2.5.1.34 dimethylallyl diphosphate + D-tryptophan 1.8% of the activity with L-tryptophan Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-D-tryptophan
-
 ir
2.5.1.34 dimethylallyl diphosphate + L-tryptophan first key step in ergot alkaloid biosynthesis providing the pharmaceutically relevant compounds lysergic acid, ergotamine, fumigaclavine A-C, festuclavine, and elymoclavine Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ir
2.5.1.34 dimethylallyl diphosphate + L-tryptophan enzyme shows high substrate specificity for dimethylallyl diphosphate and L-tryptophan Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ir
2.5.1.34 geranyl diphosphate + L-tryptophan very low activity, 0.7% of the activity with dimethylallyl diphosphate Aspergillus fumigatus diphosphate + ?
-
 ir
2.5.1.34 isopentenyl diphosphate + L-tryptophan 3.4% of the activity with dimethylallyl diphosphate Aspergillus fumigatus diphosphate + ?
-
 ir
2.5.1.34 additional information no activity with 4-hydroxyphenylpyruvate, umbelliferone, or 4-hydroxybenzoate the enzyme contains no prenyl diphosphate binding site (N/D)DXXD Aspergillus fumigatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 dimer 2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
2.5.1.34 FgaPT2
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.34 30
-
 assay at Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7.5
-
 assay at Aspergillus fumigatus