EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.39 | - |
Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.39 | additional information | enzyme disruption mutant, needs longer lag phase for growth and shows slower growth in minimal medium. Addition of alpha-aminoadipate or lysine shortens the lag phase and improves growth rate | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.39 | 0.0133 | - |
2-oxoisovalerate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 0.024 | - |
2-oxoadipate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 0.0288 | - |
2-oxoisocaproate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 0.1483 | - |
2-oxo-3-methylvalerate | pH 7.5, 45°C | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.39 | additional information | - |
sedimentation equilibrium analysis, equilibrium constant 2.4 + 10-5 M | Thermus thermophilus |
2.6.1.39 | 43845 | - |
2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
2.6.1.39 | 44000 | - |
2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.39 | Thermus thermophilus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.39 | 513 | - |
substrate phenylpyruvate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 561 | - |
substrate 2-oxo-3-methylvalerate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 568 | - |
substrate pyruvate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 661 | - |
substrate oxaloacetate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 1102 | - |
substrate 2-oxoisovalerate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 5079 | - |
substrate 2-oxoisocaproate, pH 7.5, 45°C | Thermus thermophilus |
2.6.1.39 | 6788 | - |
substrate 2-oxoadipate, pH 7.5, 45°C | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.39 | 2-oxo-3-methylvalerate + L-glutamate | - |
Thermus thermophilus | 2-amino-3-methylpentanoate + 2-oxoglutarate | - |
? | |
2.6.1.39 | 2-oxoadipate + L-glutamate | - |
Thermus thermophilus | L-2-aminoadipate + 2-oxoglutarate | - |
? | |
2.6.1.39 | 2-oxoisocaproate + L-glutamate | - |
Thermus thermophilus | L-leucine + 2-oxoglutarate | - |
? | |
2.6.1.39 | 2-oxoisovalerate + L-glutamate | - |
Thermus thermophilus | L-valine + 2-oxoglutarate | - |
? | |
2.6.1.39 | oxaloacetate + L-glutamate | - |
Thermus thermophilus | 2-aminosuccinate + 2-oxoglutarate | - |
? | |
2.6.1.39 | phenylpyruvate + 2-aminoadipate | - |
Thermus thermophilus | L-phenylalanine + 2-oxoglutarate | - |
? | |
2.6.1.39 | pyruvate + L-glutamate | - |
Thermus thermophilus | L-alanine + 2-oxoglutarate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.39 | dimer | 2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.39 | 2.1 | - |
2-oxoisovalerate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 3.5 | - |
2-oxo-3-methylvalerate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 17.8 | - |
2-oxoisocaproate | pH 7.5, 45°C | Thermus thermophilus | |
2.6.1.39 | 22.3 | - |
2-oxoadipate | pH 7.5, 45°C | Thermus thermophilus |